Overview:
ProductName | RatGRP78ELISAKit |
Description | ColorimetricdetectionofGRP78inratlysates |
SpeciesReactivity | Rat |
Platform | Microplate |
SampleTypes | Tissue |
DetectionMethod | ColorimetricAssay |
AssayType | SandwichELISA(Enzyme-linkedImmunosorbentAssay) |
Utility | ELISAkitusedtoquantitateGRP78concentrationinratsamples. |
Sensitivity | 0.15ng/ml |
AssayRange | 0-50ng/ml |
Precision | Intra-AssayPrecision:Threesamplesofknownconcentrationwereassayedthirtytimesononeplate.Theintra-assaycoefficientofvariationoftheNitrotyrosineELISAhasbeendeterminedtobe<10%. Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays. The inter-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be <15%. |
IncubationTime | 30minutes |
NumberofSamples | 40samplesinduplicate |
OtherResources | KitBooklet,MSDS |
Properties
StorageTemperature | 4ºC | ||||||||||||||||||||||||||||||||||||
ShippingTemperature | BlueIce | ||||||||||||||||||||||||||||||||||||
ProductType | ELISAKits | ||||||||||||||||||||||||||||||||||||
AssayOverview | 1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add100µLofStandardorsampletoappropriatewells.3.CoverplatewithPlateSealerandincubateat37°Cfor2hours.4.Washplatefourtimeswith1XWashBuffer.5.Add100µLofBiotinylatedAntibodyWorkingSolutiontoeachwell.6.CoverplatewithPlateSealerandincubateatat37°Cfor2hours.7.Washplatefourtimeswith1XWashBuffer.8.Add100µLofStreptavidinPolyHRPWorkingSolutiontoeachwell.9.CoverplatewithPlateSealerandincubateat37°Cfor30minutes.10.Washplatefourtimeswith1XWashBuffer.11.Add100µLofTMBSubstratetoeachwell.12.Developtheplateinthedarkatroomtemperaturefor30minutes.13.Stopreactionbyadding100µLofStopSolutiontoeachwell.14.Measureabsorbanceonaplatereaderat450nm. | ||||||||||||||||||||||||||||||||||||
KitOverview |
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CiteThisProduct | RatGRP78ELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-137) |
BIOLOGicalDescription
AlternativeNames | Grp78ELISAKit,BindingimmunoglobulinproteinELISAKit,BiPELISAKit,78kDaglucose-regulatedproteinELISAKit,heatshock70kDaprotein5ELISAKit |
ResearchAreas | Cancer,HeatShock,CellSignaling,Chaperones,OrganelleMarkers,Trafficking |
ScientificBackground | GRP78isaubiquitouslyexpressed,78-kDaglucose-regulatedprotein,andiscommonlyreferredtoasanimmunoglobinchainbindingprotein(BiP).TheBiPproteinsarecategorizedasstressresponseproteinsbecausetheyplayanimportantroleintheproperfoldingandassemblyofnascentproteinandinthescavengingofmisfoldedproteinsintheendoplasmicreticulumlumen.TranslationofBiPisdirectedbyaninternalribosomalentrysite(IRES)inthe5’nontranslatedregionoftheBiPmRNA.BiPIRESactivityincreaseswhencellsareheatstressed(1).GRP78isalsocriticalformaintenanceofcellhomeostasisandthepreventionofapoptosis(2).Luoetal.haveprovidedfindingsthatsuggestGRP78isessentialforembryoniccellgrowthandpluripotentcellsurvival(3).Intermsofdiseases,GRP78hasbeenshowntobeareliablebiomarkerofhypoglycemia,toserveaneuroprotectivefunctioninneuronsexposedtoglutamateandoxidativestress(4),anditsproteinlevelsarereducedinthebrainsofAlzheimer’spatients(5).Also,theinductionoftheGRP78proteinthatresultsinsevereglucoseandoxygendeprivationcouldpossIBLeleadtodrugresistancetoantitumordrugs(6,7). |
References | 1.ChoS.,etal.(2007).MolCellBiol27(1):368-83. 2.YangY.,etal.(1998)JBiolChem273:25552-25555. 3.LuoS.,etal(2006)26(15):5688-97. 4.YuZ.,etal.(1999)ExpNeurol.15:302-314. 5.KoomagiR.,etal.(1999)AnticancerRes.19:4333-4336. 6.LaquerreS.,etal.(1998)J.Virology72:4940-4949. 7.DongD.,etal.(2005)CancerRes65(13):5785-91. |
ProductImages
TypicalStandardCurvefortheRatGRP78ELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-137.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:0–50ng/ml.
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