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蚂蚁淘在线 / 品牌中心 / StressMarq / StressMarq/NpFR1/SIH-181-1MG/1-mg

StressMarq/NpFR1/SIH-181-1MG/1-mg

价格
￥8500.00
货号：SIH-181-1MG
浏览量：86
品牌：StressMarq
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商品描述

Overview:

ProductNameNpFR1
Description

ReversIBLefluorescenceintensity-basedredoxsensor

MolecularFormulaC23H21N5O4
MolecularWeight431.45

Properties

StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
ProductTypeRedoxProbe
SolubilitySolubleinDMSO
SourceSynthetic
AppearanceRedSolid
SMILESC1(N(C(C2=CC5=C(C3=CC=CC1=C23)N(C4=NC(NC(C4=N5)=O)=O)CCC)=O)CCCC)=O
InChIInChI=1S/C23H21N5O4/c1-3-5-10-28-21(30)13-8-6-7-12-16(13)14(22(28)31)11-15-18(12)27(9-4-2)19-17(24-15)20(29)26-23(3
InChIKeyC5(C2=C1C(=CC4=C(C1=CC=C2)N(C3=NC(NC(C3=N4)=O)=O)CCC)C(N5CCCC)=O)=O
SafetyPhrasesClassification:Caution:Substancenotyetfullytested.SafetyPhrases:S22-DonotbreathedustS24/25-AvoidcontactwithskinandeyesS36/37/39-Wearsuitableprotectiveclothing,glovesandeye/faceprotection
CiteThisProductNpFR1(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SIH-181)

BIOLOGicalDescription

ResearchAreasCancer,OxidativeStress
ScientificBackgroundNpFR1,ornapthalimide-flavinredoxsensor1,isanovelflavinmoleculethatexhibitsagreaterthan100-foldincreaseinfluorescenceuponoxidation,andiseasilyreversible.Intheoxidativeform,isshowsamaximumabsorptionat395and463nm,maxadmissionat545nm.Treatmentwithamildreducingagent(includingsodiumthiosulfate,sodiumcyanoborohydride,DTTandglutathione)gavethereducedformwhichexhibited110-foldlowerabsorbance,and125-foldloweremission.Re-oxidationtoitsoriginalfluorescencecanbeachievedbyairorbyhydrogenperoxide.
References1.YeowJ.,KaurA.K.,AnscombM.D.,andNewE.J.(2014)Chem.Commun.50:8181-8184.

ProductImages

<p>Fluorescence behavior of NpFR1 (SIH-181) in the oxidized (black) and reduced (red) forms, using 50 µM. Excitation: 405 or 488 nm (not shown). Emission: 490 – 600 nm, with peak at 545 nm.</p>

FluorescencebehaviorofNpFR1(SIH-181)intheoxidized(black)andreduced(red)forms,using50µM.Excitation:405or488nm(notshown).Emission:490–600nm,withpeakat545nm.

<p>Chemical structure of NpFR1 (SIH-181), a reversible fluorescence intensity-based redox sensor. This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).</p>

ChemicalstructureofNpFR1(SIH-181),areversiblefluorescenceintensity-basedredoxsensor.ThisimageisfromChem.Commun.,2014,50,8181,andlicensedunderaCreativeCommonsAttribution3.0UnportedLicence(http://creativecommons.org/licenses/by/3.0/).

<p>Fluorescence emission of NpFR1 (SIH-181, 5 mM, lex = 405 nm) with the incremental addition of sodium dithionite. All spectra were acquired in HEPES buffer (100 mM, pH 7.4). This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).</p>

FluorescenceemissionofNpFR1(SIH-181,5mM,lex=405nm)withtheincrementaladditionofsodiumdithionite.AllspectrawereacquiredinHEPESbuffer(100mM,pH7.4).ThisimageisfromChem.Commun.,2014,50,8181,andlicensedunderaCreativeCommonsAttribution3.0UnportedLicence(http://creativecommons.org/licenses/by/3.0/).

<p>Fluorescence response of NpFR1 (SIH-181) to cycles of oxidation and reduction. Reduction was achieved with sodium dithionite (100mM) followed by re-oxidation with 250mM H2O2. Spectra were recorded 5 min after the addition of reducing and oxidising agents. All spectra were acquired in HEPES buffer (100 mM, pH 7.4). This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).</p>

FluorescenceresponseofNpFR1(SIH-181)tocyclesofoxidationandreduction.Reductionwasachievedwithsodiumdithionite(100mM)followedbyre-oxidationwith250mMH2O2.Spectrawererecorded5minaftertheadditionofreducingandoxidisingagents.AllspectrawereacquiredinHEPESbuffer(100mM,pH7.4).ThisimageisfromChem.Commun.,2014,50,8181,andlicensedunderaCreativeCommonsAttribution3.0UnportedLicence(http://creativecommons.org/licenses/by/3.0/).

<p>Imaging of NpFR1 (SIH-181) in 3T3-L1 preadipocytes treated with (a) vehicle control (b) H2O2 (100 mM, 2 min), (c) NpFR1 (50 mM, 2 h) and (d) NpFR1 (50 mM, 2 h) followed by H2O2 (100 mM, 2 min). Scale bar represents 50 mm, lex = 405 nm. (e) Integrated emission from 510 nm to 610 nm. Values are the mean ratio generated from the intensity from five fields of cells. Error bars represent standard error measurement (s.e.m.). The layout of this image was modified for optimal display from the original Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).</p>

ImagingofNpFR1(SIH-181)in3T3-L1preADIpocytestreatedwith(a)vehiclecontrol(b)H2O2(100mM,2min),(c)NpFR1(50mM,2h)and(d)NpFR1(50mM,2h)followedbyH2O2(100mM,2min).Scalebarrepresents50mm,lex=405nm.(e)Integratedemissionfrom510nmto610nm.Valuesarethemeanratiogeneratedfromtheintensityfromfivefieldsofcells.Errorbarsrepresentstandarderrormeasurement(s.e.m.).ThelayoutofthisimagewasmodifiedforoptimaldisplayfromtheoriginalChem.Commun.,2014,50,8181,andlicensedunderaCreativeCommonsAttribution3.0UnportedLicence(http://creativecommons.org/licenses/by/3.0/).

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StressMarq细胞信号生物体中的每个细胞都暴露于来自其环境的数百种不同信号。单元可以以多种方式响应这些信号，并且独立地响应。但是，所有这些独立的动作在控制基本细胞活动的非常复杂且相互联系的通信路径中融合在一起。细胞外信号通过各种信号网络传输信息，最终在细胞核水平发挥作用。小区信令或蜂窝通信的三个阶段包括：接收信号信号转导单元内的响应 倾向于起源于质膜水平的信号传导途径或网络包括G蛋白偶联受体，（非受体）酪氨酸激酶和离子调节通道等。尽管这些过程中涉及的关键调节分子有所不同，但信号的传输通常涉及一个共同的过程。靶蛋白的可逆磷酸化。两种不同的酶介导了这一点：蛋白激酶–磷酸化蛋白质（添加磷酸基团）蛋白质磷酸酶–使蛋白质脱磷酸（去除磷酸基团）细胞感知并正确响应其微环境的能力是发育，组织修复，免疫以及正常组织动态平衡的基础。细胞内信号传导事件实际上调节了所有细胞活动。细胞信息处理中的错误可能导致癌症，自身免疫性疾病和糖尿病等疾病。通过了解细胞信号传导的基本原理，研究人员希望能够开发出新药并对其进行选择性和有效治疗。细胞信号研究需要大量的生命科学产品。我们致力于开发最先进的研究产品，以协助研究细胞信号传导，包括单克隆抗体，多克隆抗体，抗体结合物，蛋白质，免疫测定法和小分子抑制剂。