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StressMarq/MOLECULAR SIGNATURE® Nitrotyrosine ELISA Kit/SKT-126-96/96-well

价格
¥7820.00
货号:SKT-126-96
浏览量:127
品牌:StressMarq
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商品描述

Overview:

ProductNameNitrotyrosineELISAKit
Description

ColorimetricdetectionofNitrotyrosine

SpeciesReactivitySpeciesIndependent
PlatformMicroplate
SampleTypesCelllysates,Plasma,Serum,Urine
DetectionMethodColorimetricAssay
AssayTypeCompetitiveELISA(Enzyme-linkedImmunosorbentAssay)
UtilityELISAkitusedtoquantitateNitrotyrosineinsamples.
Sensitivity50nM
AssayRange62.5-8000nM
PrecisionIntra-AssayPrecision:Threesamplesofknownconcentrationwereassayedthirtytimesononeplate.Theintra-assaycoefficientofvariationoftheNitrotyrosineELISAhasbeendeterminedtobe<10%. Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays. The inter-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be <15%.
IncubationTime1hour
NumberofSamples38samplesinduplicate
OtherResourcesKitBooklet,MSDS,CalculationsWorksheet

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeELISAKits
AssayOverview1.PreparestandardandsamplesintheSampleandStandardDiluent.2.Add50µLofpreparedstandardsandsamplesintriplicatetoappropriatewells.3.Add50µLofthedilutedantibodypreparationtotheappropriatewells.4.CoverplatewithPlateCoverandincubateatroomtemperature(20-25°C)for1hour.5.Washplate4timeswith1XWashBuffer.6.Add100µLofTMBSubstratetoeachwell.7.Coverplateanddeveloptheplateinthedarkatroomtemperaturefor30minutes.8.Add100µLofStopSolutiontoeachwell.9.Measureabsorbanceonaplatereaderat450nm.10.Plotthestandardcurveandcalculatesampleconcentrations.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-126ANitrosylatedBSACoatedPlate1Plate
SKC-126CNitrotyrosineStandard1vial/110µl
SKC-126FNitrotyrosineHRPConjugatedMonoclonalAntibody1vial/75µl
SKC-0001SampleandStandardDiluent(Red)1vial/50mL
SKC-0002NitrotyrosineAntibodyDiluent(Blue)1vial/13mL
SKC-0003WashBufferConcentrate(10X)1vial/50mL
SKC-0004TMBSubstrate1vial/13mL
SKC-0005StopSolution1vial/13mL
SKC-0009PlateCover2
CiteThisProductNitrotyrosineELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-126)

BIOLOGicalDescription

AlternativeNamesNitrotyrosineELISAKit,3-NitrotyrosineELISAKit,3-Nitro-L-tyrosineELISAKit,3NitrotyrosineELISAKit,3-NTELISAKit,3NTELISAKit
ResearchAreasCancer,Alzheimer"sDisease,CellSignaling,Neurodegeneration,Neuroscience,Nitration,OxidativeStress,Parkinson"sDisease,Post-translationalModifications
ScientificBackgroundNitrotyrosinehasbeenidentifiedasaMarkerofinflammationandNOproduction.NitrotyrosineisformedinpresenceoftheactivemetaboliteNO.Variouspathwaysincludingtheformationofperoxinitriteleadtonitrotyrosineproduction.Sincenitrotyrosineisastableendproductofperoxynitriteoxidation,assessmentofitsplasmaconcentrationmaybeusefulasamarkerofNO-dependentdamageinvivo.SinceNOXisonlyanindicatorforenhancedNOproduction,proteinassociatednitrotyrosinemightbeamoresuitablemarkerfordamageinducedbyreactivenitrogenintermediatesderivedfromNO.FurThermore,mostproteinshavealongerhalflifeinthecirculationthanNOXlevels.Thepresenceofnitrotyrosinehasbeendetectedinvariousinflammatoryprocessesincludingatheroscleroticplaques,celiacdisease,rheumatoidarthritis,chronicrenalfailureandsepticshock.Innormalplasmalow,undetectable,levelsofnitrotyrosinearepresent.Nitrosylationoftheaminoacidtyrosineoccursbothforfreetyrosineandforproteinboundtyrosine.

ProductImages

<p>Typical Standard Curve for the Nitrotyrosine ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-126. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay. Assay Range: 62.5 – 8000 nM.</p>

TypicalStandardCurvefortheNitrotyrosineELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-126.AssayType:CompetitiveELISA.DetectionMethod:ColorimetricAssay.AssayRange:62.5–8000nM.

<p>Diagram of the Competitive ELISA</p>

DiagramoftheCompetitiveELISA

<p>Diagram of the Preparation of Nitrotyrosine Standards</p>

DiagramofthePreparationofNitrotyrosineStandards

<p>Diagram of the Triplicate Sample Plate Format</p>

DiagramoftheTriplicateSamplePlateFormat

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