Overview:
Product Name | Kir4.1 Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Description | Rabbit Anti-Human Kir4.1 Polyclonal | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species Reactivity | Human, Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications | WB, ICC/IF | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antibody Dilution | WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Host Species | Rabbit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen Species | Human | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen | Synthetic peptide from the mid-protein of Human Kir4.1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Concentration | 1 mg/ml | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet BiotinProperties:
Biotin Datasheet HRP (Horseradish peroxidase)Properties:
HRP Datasheet AP (Alkaline Phosphatase)Properties:
AP Datasheet
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Properties
Storage Buffer | PBS, 50% glycerol, 0.09% sodium azide |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Peptide Affinity Purified |
Clonality | Polyclonal |
Specificity | Detects 42 kDa. |
Cite This Product | StressMarq Biosciences Cat# SPC-700, RRID: AB_2705897 |
Certificate of Analysis | A 1:1000 dilution of SPC-700 was sufficient for detection of Kir4.1 in 15 µg of rat liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
Biological Description
Alternative Names | ATP sensitive inward rectifier potassium channel 10 Antibody, BIRK10 Antibody, KCNJ13 PEN Antibody, Potassium channel inwardly rectifying subfamily J member 10 Antibody, SESAME Antibody inwardly rectifying subfamily J member 10 Antibody, Inward rectifier K(+) channel Kir1.2 Antibody, Potassium channel KCNJ 10 Antibody, ATP-sensitive inward rectifier potassium channel 10 Antibody, Potassium inwardly rectifying channel subfamily J member 10 Antibody, KIR1.2 Antibody, Inwardly rectifying potassium channel Kir1.2 Antibody, ATP-dependent inwardly rectifying potassium channel Kir4.1 Antibody, Glial ATP dependent inwardly rectifying potassium channel KIR4.1 Antibody, Kcnj10 Antibody, KCNJ10 Antibody, IRK10_HUMAN Antibody, KIR4.1 Antibody, Inward rectifier K+ channel KIR1.2 Antibody, ATP dependent inwardly rectifying potassium channel Kir4.1 Antibody, Potassium channel Antibody |
Research Areas | Inward-Rectifying Potassium Channels, Ion Channels, Neuroscience, Potassium Channels |
Cellular Localization | Basolateral Cell Membrane, Membrane, Multi-Pass Membrane Protein |
Accession Number | NP_002232.2 |
Gene ID | 3766 |
Swiss Prot | P78508 |
Product Images
Western blot analysis of Rat Liver cell lysates showing detection of ~42.5 kDa Kir4.1 protein using Rabbit Anti-Kir4.1 Polyclonal Antibody (SPC-700). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Kir4.1 Polyclonal Antibody (SPC-700) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~42.5 kDa.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Kir4.1 Polyclonal Antibody (SPC-700). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Kir4.1 Polyclonal Antibody (SPC-700) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane, Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Kir4.1 Antibody. (D) Composite.
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ATTO 390 | ||
Overview:
ATTO 390 Datasheet | Optical Properties: λex = 390 nm λem = 479 nm εmax = 2.4×104 Φf = 0.90 τfl = 5.0 ns Brightness = 21.6 Laser = 365 or 405 nm |
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