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StressMarq/StressXpress® HSP70 ELISA Kit (PLANT)/SKT-124-96/96-well

价格
¥12880.00
货号:SKT-124-96
浏览量:127
品牌:StressMarq
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商品描述

Overview:

ProductNameHSP70ELISAKit(PLANT)
Description

ColorimetricdetectionusedtoquantitateHSP70inplants

SpeciesReactivityHuman,Alfalfa(Medicagosativa),Flax(Linumusitatissimum),Maize(Zeamays),Plant
PlatformMicroplate
SampleTypesCelllysates,Plantextract,Plasma,Serum,Tissue
DetectionMethodColorimetricAssay
AssayTypeSandwichELISA(Enzyme-linkedImmunosorbentAssay)
UtilityELISAkitusedtoquantitateHSP70concentrationinsamples.
Sensitivity0.18ng/ml
AssayRange1.563-100ng/ml
IncubationTime30minutes
NumberofSamples40samplesinduplicate
OtherResourcesKitBooklet,MSDS

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeELISAKits
AssayOverview1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add100µLofStandardtoappropriatewells.3.CoverplantwithPlateSealerandincubateatroomtemperaturefor1hour.4.WashplatefourtimeswithWashBuffer.5.Add100µLofDetectionAntibodyWorkingSolutiontoeachwell.6.CoverplatewithPlateSealerandincubateatroomtemperaturefor1hour.7.Washplatefourtimeswith1XWashBufferasdescribedinstep4.8.Add100µLofStreptavidin-HRPWorkingSolutiontoeachwell.9.CoverplatewithPlateSealerandincubateatroomtemperaturefor30minutes.10.Washplatefourtimeswith1XWashBufferasdescribedinstep4.11.Add100µLofTMBSubstratetoeachwell.12.Developtheplateinthedarkatroomtemperaturefor30minutes.13.Stopreactionbyadding100µLofStopSolutiontoeachwell.14.Measureabsorbanceonaplatereaderat450nm.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-124AAnti-Hsp70ImmunoassayPlate1Plate
SKC-124B5XHsp70ExtractionReagent1vial/10ml
SKC-124CRecombinantHsp70(Alfalfa)Standard2vials
SKC-124DStandardandSampleDiluent1vial/50ml
SKC-124E10XWashBufferConcentrate1vial/100ml
SKC-124FAnti-Hsp70BiotinylatedAntibodyConcentrate1vial/150µl
SKC-124GAnti-Hsp70BiotinylatedAntibodyDiluent1vial/13ml
SKC-124HStreptavidin:HRPConcentrate1vial/50µl
SKC-124IStreptavidin:HRPDiluent1vial/13ml
SKC-124JTMBSubstrate1vial/13ml
SKC-124KStopSolution1vial/13ml
CiteThisProductHSP70ELISAKit(PLANT)(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-124)

BIOLOGicalDescription

AlternativeNamesHSP701ELISAKit,HSP702ELISAKit,HSP70.1ELISAKit,HSP72ELISAKit,HSPA1ELISAKit,HSPA1AELISAKit,HSPA1BELISAKit
ResearchAreasCancer,HeatShock
ScientificBackgroundHSP70genesencodeabundantheat-inducIBLe70-kDaHSPs(HSP70s).InmosteukaryotesHSP70genesexistaspartofamultigenefamily.Theyarefoundinmostcellularcompartmentsofeukaryotesincludingnuclei,mitochondria,chloroplasts,theendoplasmicreticulumandthecytosol,aswellasinbacteria.Thegenesshowahighdegreeofconservation,havingatleast50%identity(2).TheN-terminaltwothirdsofHSP70saremoreconservedthantheC-terminalthird.HSP70bindsATPwithhighaffinityandpossessesaweakATPaseactivitywhichcanbestimulatedbybindingtounfoldedproteinsandsyntheticpeptides(3).WhenHSC70(constitutivelyexpressed)presentinmammaliancellswastruncated,ATPbindingactivitywasfoundtoresideinanN-terminalfragmentof44kDawhichlackedpeptidebindingcapacity.PolypeptidebindingABIlitythereforeresidedwithintheC-terminalhalf(4).ThestructureofthisATPbindingdomaindisplaysmultiplefeaturesofnucleotidebindingproteins(5).AllHSP70s,regardlessoflocation,bindproteins,particularlyunfoldedones.ThemolecularchaperonesoftheHSP70familyrecognizeandbindtonascentpolypeptidechainsaswellaspartiallyfoldedintermediatesofproteinspreventingtheiraggregationandmisfolding.ThebindingofATPtriggersacriticalconformationalchangeleADIngtothereleaseoftheboundsubstrateprotein(6).TheuniversalabilityofHSP70stoundergocyclesofbindingtoandreleasefromhydrophobicstretchesofpartiallyunfoldedproteinsdeterminestheirroleinagreatvarietyofvitalintracellularfunctionssuchasproteinsynthesis,proteinfoldingandoligomerizationandproteintransport.LookingformoreinformationonHSP70?VisitournewHSP70ScientificResourceGuideathttp://www.HSP70.com.
References1.BoorsteinW.R.,ZiegelhofferT.&CraigE.A.(1993)J.Mol.Evol.38(1):1-17.
2.RothmanJ.(1989)Cell.59:591-601.
3.DeLuca-Flahertyetal.(1990)Cell.62:875-887.
4.BorkP.,SanderC.&ValenciaA.(1992)Proc.NatlAcad.Sci.USA.89:7290-7294.
5.FinkA.L.(1999)Physiol.Rev.79:425-449.

ProductImages

<p>Typical Standard Curve for the HSP70 ELISA Kit (PLANT) (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-124. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 1.563 – 100 ng/mL.</p>

TypicalStandardCurvefortheHSP70ELISAKit(PLANT)(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-124.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:1.563–100ng/mL.

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