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StressMarq/StressXpress® HSP65 ELISA Kit/SKT-113-96/96-well

价格
¥11220.00
货号:SKT-113-96
浏览量:127
品牌:StressMarq
服务
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商品描述

Overview:

ProductNameHSP65ELISAKit
Description

ColorimetricdetectionofHSP65

SpeciesReactivityMycobacteriumBovis,MycobacteriumTuberculosum
PlatformMicroplate
SampleTypesCelllysates,Serum,Tissue
DetectionMethodColorimetricAssay
AssayTypeSandwichELISA(Enzyme-linkedImmunosorbentAssay)
UtilityELISAkitusedtoquantitateM.bovis/M.tuberculosumHSP65concentrationinhumansamples
Sensitivity0.06ng/ml
AssayRange0.344-22ng/ml
IncubationTime30minutes
NumberofSamples40samplesinduplicate
OtherResourcesKitBooklet,MSDS

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeELISAKits
AssayOverview1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add100µLofStandardorsampletoappropriatewells.3.CoverplatewithPlateSealerandincubateatroomtemperature(20-25°C)for1hour.4.Washplatefourtimeswith1XWashBuffer.5.Add100µLofBiotinylatedAntibodyWorkingSolutiontoeachwell.6.CoverplatewithPlateSealerandincubateatroomtemperaturefor1hour.7.Washplatefourtimeswith1XWashBuffer.8.Add100µLofStreptavidin-HRPWorkingSolutiontoeachwell.9.CoverplatewithPlateSealerandincubateatroomtemperaturefor30minutes.10.Washplatefourtimeswith1XWashBuffer.11.Add100µLofTMBSubstratetoeachwell.12.Developtheplateinthedarkatroomtemperaturefor30minutes.13.Stopreactionbyadding100µLofStopSolutiontoeachwell.14.Measureabsorbanceonaplatereaderat450nm.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-113AAnti-Hsp65ImmunoassayPlate1Plate
SKC-113B5XHsp65ExtractionReagent1vial/10ml
SKC-113CRecombinantHsp65Standard2vials
SKC-113DStandardandSampleDiluent1vial/50ml
SKC-113E10XWashBufferConcentrate1vial/100ml
SKC-113FAnti-Hsp65BiotinylatedAntibodyConcentrate1vial/150µl
SKC-113GAnti-Hsp65BiotinylatedAntibodyDiluent1vial/13ml
SKC-113HStreptavidin:HRPConcentrate1vial/150µl
SKC-113IStreptavidin:HRPDiluent1vial/13ml
SKC-113JTMBSubstrate1vial/13ml
SKC-113KStopSolution1vial/13ml
CiteThisProductHSP65ELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-113)

BIOLOGicalDescription

AlternativeNames60kDachaperonin2ELISAKit,CellwallproteinAELISAKit,groELProtein2ELISAKit,GroEL2ELISAKit,M.TuberculosiscellwallproteinAELISAKit,M.TuberculosisHSP65ELISAKit
ResearchAreasCancer,HeatShock
ScientificBackgroundHSP65isamemberoftheHSP60familyofheatshockproteins(2,3).HSP60saremitochondrialchaperoninsthataretypicallyheldresponsIBLeforthetransportationandrefoldingofproteinsfromthecytoplasmintothemitochondrialmatrix.Inadditiontoitsroleasaheatshockprotein,HSP60functionsasachaperonintoassistinfoldinglinearaminoacidchainsintotheirrespectivethree-dimensionalstructure.HSP60sareaubiquitousclassofHSPsthatspecificallypromotethefoldingandassemblyofcellularpolypeptidesinanATP-dependentmanner(1).Specifically,sequencecomparisonofHSP65fromdifferentmycobacteriumstrainsshowedthattheproteinsequenceofM.bovisBCGisidenticaltothatofM.tuberculosis,andverysimilartothatofM.leprae,thepathogensthatcausetuberculosisandtuberculoidleprosy,respectively(2,4).MycobacteriumbovisBCGHSP65wasidentifiedastheimmunodominantantigenduringmycobacterialdiseasesandvaccination.Itisalsobelievedtobetheantigenthatinducesautoimmunedisease,suchasadjuvantarthritisinrats(5,6).
References1.KollH.,etal.(1992)Cell.68:1163-1175.
2.TholeJ.E.R.,etal.(1985)Infect.Immuno.50:800-806.
3.TholeJ.E.R.,etal.,(1987)Infect.Immuno.55:1466-1475.
4.ShinnickT.M.SweetserD.,TholeJ.,vanEmBDenJ.andYoungR.A.(1987)Infect.Immuno.55:1932-1935.
5.VanEdenW.,etal.(1988)Nature331:171-178.
6.CobelensP.M.,etal.(2002)Rheumatology41:775-779.

ProductImages

<p>Typical Standard Curve for the HSP65 ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-113. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.344 – 22 ng/mL.</p>

TypicalStandardCurvefortheHSP65ELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-113.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:0.344–22ng/mL.

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