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StressMarq/StressXpress® HSP60 ELISA Kit/SKT-110-96/96-well

价格
¥10280.00
货号:SKT-110-96
浏览量:127
品牌:StressMarq
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商品描述

Overview:

ProductNameHSP60ELISAKit
Description

ColorimetricdetectionofHSP60

SpeciesReactivityHuman
PlatformMicroplate
SampleTypesCelllysates,Serum,Tissue
DetectionMethodColorimetricAssay
AssayTypeSandwichELISA(Enzyme-linkedImmunosorbentAssay)
UtilityELISAkitusedtoquantitateHSP60concentrationinsamples.
Sensitivity1.73ng/ml
AssayRange11-700ng/ml
IncubationTime30minutes
NumberofSamples40samplesinduplicate
OtherResourcesKitBookletLotNo.BH585599,KitBookletLotNo.TH585578,KitBookletLotNo.160427,MSDS

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeELISAKits
AssayOverview1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add100µLofStandardorsampletoappropriatewells.3.CoverplatewithPlateSealerandincubateat37°Cfor1hour.4.Washplatefourtimeswith1XWashBuffer.5.Add100µLofBiotinylatedAntibodyWorkingSolutiontoeachwell.6.CoverplatewithPlateSealerandincubateat37°Cfor1hour.7.Washplatefourtimeswith1XWashBuffer.8.Add100µLofStreptavidin-HRPWorkingSolutiontoeachwell.9.CoverplatewithPlateSealerandincubateatroomtemperaturefor30minutes.10.Washplatefourtimeswith1XWashBuffer.11.Add100µLofTMBSubstratetoeachwell.12.Developtheplateinthedarkatroomtemperaturefor30minutes.13.Stopreactionbyadding100µLofStopSolutiontoeachwell.14.Measureabsorbanceonaplatereaderat450nm.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-110AAnti-Hsp60ImmunoassayPlate1Plate
SKC-110B5XHsp60ExtractionReagent1vial/10ml
SKC-110CRecombinantHsp60Standard1vial
SKC-110DStandardandSampleDiluent1vial/50ml
SKC-110E10XWashBufferConcentrate1vial/100ml
SKC-110FAnti-Hsp60BiotinylatedAntibodyConcentrate1vial/150µl
SKC-110GAnti-Hsp60BiotinylatedAntibodyDiluent1vial/13ml
SKC-110HStreptavidin:HRPConcentrate1vial/150µl
SKC-110IStreptavidin:HRPDiluent1vial/13ml
SKC-110JTMBSubstrate1vial/13ml
SKC-110KStopSolution1vial/13ml
CiteThisProductHSP60ELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-110)

BIOLOGicalDescription

AlternativeNamesCPN60ELISAKit,GROELELISAKit,HLD4ELISAKit,HSP60ELISAKit,HSP65ELISAKit,HSPD1ELISAKit,HuCHA60ELISAKit,SPG13ELISAKit
ResearchAreasCancer,HeatShock
ScientificBackgroundInbothprokaryoticandeukaryoticcells,themisfoldingandaggregationofproteinsduringbiogenesisandunderconditionsofcellularstressarepreventedbymolecularchaperones.MembersoftheHSP60familyofheatshockproteinsaresomeofthebestcharacterizedchaperones.HSP60,alsoknownasCpn60orGroEl,isanabundantproteinsynthesizedconstitutivelyinthecellthatisinducedtoahigherconcentrationafterbriefcellshock.Itispresentinmanyspeciesandexhibitsaremarkablesequencehomologyamongvariouscounterpartsinbacteria,plants,andmammalswithmorethanhalfoftheresiduesidenticalbetweenbacterialandmammalianHSP60(1-3).WhereasmammalianHSP60islocalizedwithinthemitochondria,plantHSP60,orotherwiseknownasRubisco-bindingprotein,islocatedinplantchloroplasts.IthasbeenindicatedthattheseproteinscarryoutaveryimportantbiologicalfunctionduetothefactthatHSP60ispresentinsomanydifferentspecies.ThecommoncharacteristicsoftheHSP60sfromthedivergentspeciesarei)highabundance,ii)inductionwithenvironmentalstresssuchasheatshock,iii)homo-oligomericstructuresofeither7or14subunitswhichreversIBLydissociateinthepresenceofMg2+andATP,iv)ATPaseactivityandv)aroleinfoldingandassemblyofoligomericproteinstructures(4).Thesesimilaritiesaresupportedbyrecentstudieswherethesingle-ringhumanmitochondrialhomolog,HSP60withitsco-chaperonin,HSP10wereexpressedinaE.colistrain,engineeredsothatthegroEoperonisunderstrictregulatorycontrol.ThisstudyhasdemonstratedthatexpressionofHSP60-HSP10wasabletocarryoutallessentialinvivofunctionsofGroELanditsco-chaperonin,GroES(5).AnotherimportantfunctionofHSP60andHSP10istheirprotectivefunctionsagainstinfectionandcellularstress.HSP60hashoweverbeenlinkedtoanumberofautoimmunediseases,aswellasAlzheimers,coronaryarterydiseases,MS,anddiabetes(6-9).
References1.HartlF.U.(1996)Nature.381:571-579.
2.BukauB.andHorwich,A.L.(1998)Cell.92:351-366.
3.HartlF.U.andHayer-Hartl,M.(2002)Science.295:1852-1858.
4.JindalS.,etal.(1989)MolecularandCellularBiology.9:2279-2283.
5.LaVerda,D.,etal(1999)InfectDis.Obstet.Gynecol.7:64-71.
6.ItohH.,etal.(2002)Eur.J.Biochem.269:5931-5938.
7.GuptaS.andKnowltonA.A.J.CellMol.Med.9:51-58.
8.DeocarisC.C.,etal.(2006)CellStressChaperones.11:116-128.
9.LaiH.C.,etal.(2007)Am.J.Physiol.Endocrinol.Metab.292:E292-E297.

ProductImages

<p>Typical Standard Curve for the HSP60 ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-110. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 7 – 450 ng/mL.</p>

TypicalStandardCurvefortheHSP60ELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-110.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:7–450ng/mL.

<p>Typical Standard Curve for the HSP60 ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-110. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 11 – 700 ng/ml.</p>

TypicalStandardCurvefortheHSP60ELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-110.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:11–700ng/ml.

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