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StressMarq/StressXpress® HO-1 ELISA Kit/SKT-111-96/96-well

价格
¥10280.00
货号:SKT-111-96
浏览量:127
品牌:StressMarq
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商品描述

Overview:

ProductNameHO-1ELISAKit
Description

Colorimetricdetectionofhemeoxygenase1

SpeciesReactivityHuman
PlatformMicroplate
SampleTypesCelllysates,Serum,Tissue
DetectionMethodColorimetricAssay
AssayTypeSandwichELISA(Enzyme-linkedImmunosorbentAssay)
UtilityELISAkitusedtoquantitateHO-1concentrationinsamples.
Sensitivity0.21ng/ml
AssayRange0.781-50ng/mL
IncubationTime30minutes
NumberofSamples40samplesinduplicate
OtherResourcesKitBooklet,MSDS

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeELISAKits
AssayOverview1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add50µLofPre-TreatmentBuffertoallsampleandstandardwells.3.Add50µLofStandardandsampletoappropriatewells.4.CoverplatewithPlateSealerandincubateatroomtemperature(20-25°C)for2hours.5.Washplatefourtimeswith1XWashBuffer.6.Add100µLofBiotinylatedAntibodyWorkingSolutiontoeachwell.7.CoverplatewithPlateSealerandincubateatroomtemperaturefor1hour.8.Washplatefourtimeswith1XWashBufferasdescribedinstep5.9.Add100µLofStreptavidin-HRPWorkingSolutiontoeachwell.10.CoverplatewithPlateSealerandincubateatroomtemperaturefor30minutes.11.Washplatefourtimeswith1XWashBufferasdescribedinstep5.12.Add100µLofTMBSubstratetoeachwell.13.Developtheplateinthedarkatroomtemperaturefor30minutes.14.Stopreactionbyadding100µLofStopSolutiontoeachwell.15.Measureabsorbanceonaplatereaderat450nm.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-111AAnti-HO-1ImmunoassayPlate1Plate
SKC-111B5XHO-1ExtractionReagent1vial/10ml
SKC-111CRecombinantHO-1Standard2vials
SKC-111DStandardandSampleDiluent1vial/50ml
SKC-111E10XWashBufferConcentrate1vial/100ml
SKC-111FAnti-HO-1BiotinylatedAntibodyConcentrate1vial/150µl
SKC-111GAnti-HO-1BiotinylatedAntibodyDiluent1vial/13ml
SKC-111HStreptavidin:HRPConcentrate1vial/150µl
SKC-111IStreptavidin:HRPDiluent1vial/13ml
SKC-111JTMBSubstrate1vial/13ml
SKC-111KStopSolution1vial/13ml
SKC-111LPre-treatmentBuffer1vial/13ml
CiteThisProductHO-1ELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-111)

BIOLOGicalDescription

AlternativeNamesHemeoxygenase1ELISAKit,HemoxELISAKit,HMOX1ELISAKit,HO1ELISAKit,HO1ELISAKit,HSP32ELISAKit
ResearchAreasCancer,OxidativeStress
ScientificBackgroundHeme-oxygenaseisaubiquitousenzymethatcatalyzestheinitialandrate-limitingstepsinhemecatabolismyieldingequimolaramountsofbiliverdin,ironandcarbonmonoxide.Biliverdinissubsequentlyconvertedtobilirubinandthefreeironissequesteredtoferritin(1).Theseproductshaveimportantphysiologicaleffectsascarbonmonoxideisapotentvasodilator;biliverdinandbilirubinarepotentantioxidants;andthefreeironincreasesoxidativestressandregulatestheexpressionofmanymRNAs(2).Therearethreeisoformsofheme-oxygenase,HO-1,HO-2andHO-3;howeverHO-1andHO-2arethemajorisoformsastheybothhavebeenidentifiedinmammals(3).HO-1,alsoknownasheatshockprotein32,isaninducIBLeisoformactivatedbymostoxidativestressinducers,cytokines,inflammatoryagentsandheatshock.HO-2isaconstitutiveisoformwhichisexpressedunderhomeostaticconditions.HO-1isalsoconsideredtobeacytoprotectivefactorinthatfreehemeishighlyreactiveandcytotoxic,andsecondly,carbonmonoxideisamediatorinhibitingtheinflammatoryprocessandbilirubinisascavengerforreactiveoxygen,bothofwhicharetheendproductsofhemecatalyzation(4).IthasalsobeenshownthatHO-1deficiencymaycausereducedstressdefense,apro-inflammatorytendency(5),susceptibilitytoatheroscleroticlesionformation(6),endothelialcellinjury,andgrowthretardation(7).Up-regulationofHO-1isthereforesaidtobeoneofthemajordefensemechanismsofoxidativestress(4).
References1.FrohM.etal.(2007)WorldJ.Gastroentereol13(25):3478-86.
2.ElbirtK.K.andBonkovskyH.L.(1999)ProcAssocAmPhysicians111(5):348-47.
3.MainesM.D.,TrakshelG.M.,andKuttyR.K.(1986)JBiolChem261:411–419.
4.BrydunA.,etal.(2007)HypertensRes30(4):341-8.
5.PossK.D.andTonegawaS.(1997).ProcNatlAcadSciUSA.94:10925–10930.
6.YetS.F.,etal.(2003)FASEBJ.17:1759–1761.
7.YachieA.,etal.(1999)JClinInvest.103:129–135.

ProductImages

<p>Typical Standard Curve for the HO-1 ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-111. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.781 – 50 ng/mL.</p>

TypicalStandardCurvefortheHO-1ELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-111.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:0.781–50ng/mL.

ProductCitations(1)

OtherCitations

Protectiveeffectofremotelimbpostconditioningviaupregulationofheme-oxygenase-1/BDNFpathwayinratmodelofcerebralischemicreperfusioninjury.

Ramagiri,S.andTaliyan,R.(2017)BrainRes.[Epubaheadofprint].

PubMedID:28535981ReactivityRatApplications:Brain

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