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StressMarq/StressXpress® Cystatin C EIA Kit/SKT-219-96/96-well

价格
¥8480.00
货号:SKT-219-96
浏览量:127
品牌:StressMarq
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商品描述

Overview:

ProductNameCystatinCEIAKit
Description

ColorimetricmeasurementofhumancystatinClevels

SpeciesReactivityHuman
PlatformMicroplate
SampleTypesEDTAPlasma,HeparinPlasma,Serum,TissueCultureMedia,Urine
DetectionMethodColorimetricAssay
AssayTypeSandwichEIA(EnzymeImmunoassay)
UtilityEIAkitusedtomeasurecystatinClevelsinsamples.
Sensitivity0.058ng/ml
AssayRange0.156-10ng/ml
PrecisionIntraAssayPrecision:FourhumansamplesweredilutedwithAssayBufferandruninreplicatesof20inanassay.ThemeanandprecisionofthecalculatedCystatinCconcentrationswere:Sample1-7.72ng/mL,9.1%CVSample2-5.08ng/mL,10.3%CVSample3-4.42ng/mL,7.6%CVSample4-0.88ng/mL,10.3%CVInterAssayPrecision:FourhumansamplesweredilutedwithAssayBufferandruninduplicatesintenassaysrunovermultipledaysbythreeoperators.ThemeanandprecisionofthecalculatedCystatinCconcentrationswere:Sample1-7.79ng/mL,8.4%CVSample2-5.48ng/mL,10.2%CVSample3-4.97ng/mL,11.1%CVSample4-1.04ng/mL,12.40%CV
IncubationTime2hours
NumberofSamples40samplesinduplicate
OtherResourcesKitBooklet,MSDS

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeEIAKits
AssayOverviewTheCystatinCEIAkitisdesignedtoquantitativelymeasurehumanCystatinCpresentinBIOLOGicalsamplesandtissueculturemedia.AhumanCystatinCstandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.StandardsordilutedsamplesarePipettedintoaclearmicrotiterplatecoatedwithamonoclonalantibodytocapturetheCystatinCpresent.Aftera60minuteincubation,theplateiswashedandaperoxidaseconjugatedCystatinCmonoclonalantibodyisadded.Theplateisagainincubatedfor30minutesandwashed.Substrateisthenaddedtotheplate,whichreactswiththeboundCystatinCAntibodyConjugate.Afterathirdincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength.TheconcentrationoftheCystatinCinthesampleiscalculated,aftermakingsuitablecorrectionfordilution,usingsoftwareavailablewithmostplatereaders.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-219AClear96wellPlate1Plate
SKC-219BCystatinCStandard60µl
SKC-219CStressXpress®CystatinCConjugate5ml
SKC-219DAssayBufferConcentrate28ml
SKC-219EWashBufferConcentrate30ml
SKC-219FTMBSubstrate5ml
SKC-219GStopSolution5ml
SKC-219HPlateSealer1Each
CiteThisProductCystatinCEIAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-219)

BiologicalDescription

AlternativeNamesCystatin3EIAKit,gammatraceEIAKit,post-gamma-globulinorneuroendocrinebasicpolypeptideEIAKit
ResearchAreasCancer,Alzheimer"sDisease,CellSignaling,Neurodegeneration,Neuroscience
ScientificBackgroundCystatinCisanon-glycosylatedproteinoflowmolecularweight(13kDa)inthecystatinsuperfamily.CystatinCisproducedataconstantrateinallnucleatedcells,secretedfromcellsandthusfoundindetectableamountsinmostbodyfluids(1,2).CystatinCbelongstothecysteineproteinaseinhibitorgroupandisassociatedwithseveralpathologicalconditions.ImbalancebetweenCystatinCandcysteineproteinasesisassociatedwithdiseasessuchasinflammation,renalfailure,cancer,Alzheimer’sdisease,multiplesclerosisandhereditaryCystatinCamyloidangiopathy(1,3,4).Increasedlevelshavebeenfoundinautoimmunediseases,withcolorectaltumorsandmetastases,patientswithinflammationandinpatientsondialysis(5,6,7).CystatinCisremovedfrombloodplasmabyglomerularfiltrationinthekidneys.Itisreabsorbedbytheproximaltubularcellsanddegraded.ThereisalinearrelationshipbetweenthereciprocalCystatinCconcentrationinplasmaandtheglomerularfiltrationrate(GFR).CystatinCissuggestedtobeabetterMarkerforGFRthantheubiquitousserumcreatininemarkerasitsserumconcentrationisnotaffectedbyotherfactorssuchasage,genderandbodymassandCystatinChashighersensitivitytodetectareducedGFRthancreatininedetermination(2,5).LowlevelsofCystatinCarefoundwiththebreakdownoftheelasticlaminaeandatherosclerosisandaBDominalaorticaneurysm(8).ThereisevidentassociationofCystatinClevelswiththeincidenceofmyocardialinfarction,coronarydeathandanginapectoris,presentingariskfactorforsecondarycardiovascularevents(9).
References1.PergandeM.andJungK.ClinChem.199339(9):1885-90.
2.NewmanD,etal.KidneyInt’l.1995Jan;47(1):312-8.
3.LevyE.ExpertRev.Neurotherapeutics2008May;8(5):687-9.
4.NakashimaI,etal.AnnNeurol.2007Aug;62(2):197-200.
5.UchidaK.andGotoh,A.ClinChimActa.2002Sep;323:121-8.
6.PucciL,etal.ClinChem.2007Mar;53(3):480-8.
7.StabucB,etal.ClinChem.2000Feb;46(2):193-7.
8.ShlipakM,etal.NEJM2005May;352(20):2049-60.
9.KoenigW,etal.ClinChem.2005Feb;51(2):321-7.

ProductImages

<p>Typical Standard Curve for the Cystatin C EIA Kit (Enzyme Immunoassay) StressXpress® – SKT-219. Assay Type: Sandwich EIA. Detection Method: Colorimetric Assay. Assay Range: 0.156 – 10 ng/ml.</p>

TypicalStandardCurvefortheCystatinCEIAKit(EnzymeImmunoassay)StressXpress®–SKT-219.AssayType:SandwichEIA.DetectionMethod:ColorimetricAssay.AssayRange:0.156–10ng/ml.

<p>Serum linearity was determined by taking two human serum samples diluted 1:100, one with a low diluted Cystatin C level of 4.94 ng/mL and one with a higher diluted level of 12.31 ng/mL and mixing them in the ratios given below. Urine linearity samples were diluted 1:16. One sample with a value of 1.64 ng/mL was mixed in ratios with a sample of 3.72 ng/mL. The diluted serum and urine samples were mixed 4:1, 3:2, 2:3 and 1:4 and run in the assay. The measured concentrations were compared to the values previously determined.</p>

Serumlinearitywasdeterminedbytakingtwohumanserumsamplesdiluted1:100,onewithalowdilutedCystatinClevelof4.94ng/mLandonewithahigherdilutedlevelof12.31ng/mLandmixingthemintheratiosgivenbelow.Urinelinearitysampleswerediluted1:16.Onesamplewithavalueof1.64ng/mLwasmixedinratioswithasampleof3.72ng/mL.Thedilutedserumandurinesamplesweremixed4:1,3:2,2:3and1:4andrunintheassay.Themeasuredconcentrationswerecomparedtothevaluespreviouslydetermined.

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