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苏州工业园区生物纳米园A4#216
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0512-67156496
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商品描述
Overview:
| ProductName | CyclicGMPEIAKit |
| Description | QuantitativecolorimetricdetectionofcGMP |
| SpeciesReactivity | SpeciesIndependent |
| Platform | Microplate |
| SampleTypes | Celllysates,EDTAPlasma,Saliva,Tissue,TissueCultureMedia,Urine |
| DetectionMethod | ColorimetricAssay |
| AssayType | DirectEIA(EnzymeImmunoassay) |
| Utility | EIAkitusedtoquantitativelymeasurecGMPpresentinsamples. |
| Sensitivity | 0.28pmol/ml |
| AssayRange | Regular:0.5-32pmol/ml.Acetylated:0.3125-20pmol/ml |
| Precision | IntraAssayPrecision(Regular):ThreehumanurinesamplesweredilutedwithSampleDiluentandruninreplicatesof20inanassay.ThemeanandprecisionofthecalculatedcGMPconcentrationswere:Sample1-8.5pmol/mL,6.3%CVSample2-4.0pmol/mL,7.8%CVSample3-1.2pmol/mL,13.6%CVIntraAssayPrecision(Acetylated):ThreehumanurinesamplesweredilutedwithSampleDiluent,acetylatedandruninreplicatesof20inanassay.ThemeanandprecisionofthecalculatedcGMPconcentrationswere:Sample1-8.1pmol/mL,3.8%CVSample2-3.8pmol/mL,5.2%CVSample3-1.2pmol/mL,8.2%CVInterAssayPrecision(Regular):ThreehumanurinesamplesweredilutedwithSampleDiluentandruninduplicatesintwelveassaysrunovermultipledaysbythreeoperators.ThemeanandprecisionofthecalculatedcGMPconcentrationswere:Sample1-8.5pmol/mL,6.5%CVSample2-4.3pmol/mL,7.0%CVSample3-1.5pmol/mL,8.3%CVInterAssayPrecision(Acetylated):ThreehumanurinesampleweresdilutedwithSampleDiluent,acetylatedandruninduplicatesintwelveassaysrunovermultipledaysbythreeoperators.ThemeanandprecisionofthecalculatedcGMPconcentrationswere:Sample1-8.7pmol/mL,12.3%CVSample2-4.3pmol/mL,14.3%CVSample3-1.5pmol/mL,14.2%CV |
| NumberofSamples | 39samplesinduplicate |
| OtherResources | KitBooklet,MSDS,SalivaSampleHandlingInstructions |
Properties
| StorageTemperature | 4ºC | |||||||||||||||||||||||||||||||||||||||
| ShippingTemperature | BlueIce | |||||||||||||||||||||||||||||||||||||||
| ProductType | EIAKits | |||||||||||||||||||||||||||||||||||||||
| AssayOverview | TheCyclicGMP(cGMP)EIAkitisdesignedtoquantitativelymeasurecGMPpresentinlysedcells,EDTAplasma,urine,salivaandtissueculturemediasamples.ForsampleswherethelevelsofcGMPareexpectedtoberelativelyhigh,theregularformatfortheassaycanbeused.ForsampleswithexpectedlowlevelsofcGMP,anoptionalacetylationprotocolcanbeused.ThekitisuniqueinthatallsamplesandstandardsaredilutedintoanacidicSampleDiluent,whichcontainsspecialadditivesandstABIlizers,forcGMPmeasurement.Thisallowsplasma,urineandsalivasamplestobereadinanidenticalmannertolysedcells.AcidifiedsamplesofcGMParestableandendogenousphosphodiesterasesareinactivatedintheSampleDiluent.AcGMPstandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.AclearmicrotiterplatecoatedwithanantibodytocapturemouseIgGisprovidedandaneutralizingPlatePrimersolutionisaddedtoalltheusedwells.Standardsordilutedsamples,eitherwithorwithoutacetylation,arePipettedintotheprimedwells.AcGMP-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.ThebindingreactionisinitiatedbytheadditionofamousemonoclonalantibodytocGMPtoeachwell.Aftera2hourincubation,theplateiswashedandsubstrateisadded.ThesubstratereactswiththeboundcGMP-peroxidaseconjugate.Afterashortincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength.TheconcentrationofthecGMPinthesampleiscalculated,aftermakingsuitablecorrectionforthedilutionofthesample,usingsoftwareavailablewithmostplatereaders. | |||||||||||||||||||||||||||||||||||||||
| KitOverview |
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| CiteThisProduct | CyclicGMPEIAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-211) |
BIOLOGicalDescription
| AlternativeNames | cGMP;3",5"-cyclicGMPEIAKit,GuanosinecyclicmonophosphateEIAKit,Cyclic3",5"-GMP;Guanosine3",5"-cyclicphosphateEIAKit |
| ResearchAreas | CellSignaling,Neuroscience |
| ScientificBackground | Guanosine3’,5’-cyclicmonophosphate(cyclicGMP;cGMP)isacriticalandmultifunctionalsecondmessengerpresentatlevelstypically10-100foldlowerthancAMPinmosttissues.IntracellularcGMPisformedbytheactionoftheenzymeguanylatecyclaseonGTPanddegradedthroughphosphodiesterasehydrolysis(1-3).Guanylatecyclases(GC)areeithersolubleormembranebound(3,4).SolubleGCsarenitricoxideresponsive,whereasmembraneboundGCsrespondtohormonessuchasacetylcholine,insulinandoxytocin.OtherchemicalslikeSEROtoninandhistaminealsocauseanincreaseincGMPlevels(5,6).CyclicGMPregulatescellularcompositionthroughcGMP-dependentkinase,cGMP-dependentionchannelsortransporters,andthroughitshydrolyticdegradationbyphosphodiesterase(1,7) |
| References | 1.Domek-Lopacinska,K.andStrosznajder,JB.(2005)JPhysiolPharmacol56Suppl2,15-34. 2.Lucas,K.A.etal.(2000)PharmacolRev52:375-414. 3.Ashman,DF.,etal.(1963),BiochemBiophysResComm,11:330-4. 4.PotterLR,Abbey-HoschS,andDickeyDM.(2006)EndocrRev27:47-72. 5.Waldman,SAandMurad,F.(1987)PharmacolRevs,39:163-197. 6.TremblayJ,GerzerR,andHametP.(1988),Adv.2ndMessanger&PhosphoproteinRes.,22:319-383. 7.MatsumotoT,KobayashiT,andKamataK.(2003)JSmoothMuscleRes39:67-86. |
ProductImages
RegularFormatTypicalStandardCurvefortheCyclicGMPEIAKit(EnzymeImmunoassay)StressXpress®–SKT-211.AssayType:DirectEIA.DetectionMethod:ColorimetricAssay.AssayRange:Regular:0.5–32pmol/ml.Acetylated:0.3125–20pmol/ml.
AcetylatedFormatTypicalStandardCurvefortheCyclicGMPEIAKit(EnzymeImmunoassay)StressXpress®–SKT-211.AssayType:DirectEIA.DetectionMethod:ColorimetricAssay.AssayRange:Regular:0.5–32pmol/ml.Acetylated:0.3125–20pmol/ml.
Linearitywasdeterminedbytakingtwohumanurinesamples,onewithalowcGMPlevelof0.3pmol/mLandonewithahigherlevelof9.7pmol/mL,andmixingtheminratios.Themeasuredconcentrationswerecomparedtotheexpectedvaluesbasedontheratiosused.
ChemicalstructureofcGMP(3′,5′-cyclicGMP)
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