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蚂蚁淘在线 / 品牌中心 / StressMarq / StressMarq/StressXpress® Cyclic GMP CLIA Kit (High-Sensitivity)/SKT-210-96/96-well
商品描述
Overview:
| ProductName | CyclicGMPCLIAKit(High-Sensitivity) |
| Description | ChemiluminescentdetectionofcGMP |
| SpeciesReactivity | SpeciesIndependent |
| Platform | Microplate |
| SampleTypes | Celllysates,EDTAPlasma,Saliva,Tissue,TissueCultureMedia,Urine |
| DetectionMethod | ChemiluminescentAssay |
| AssayType | DirectCLIA(ChemiluminescentImmunoassay) |
| Utility | CLIAkitusedtomeasurecGMPpresentinsamples. |
| Sensitivity | 0.034pmol/ml |
| AssayRange | Regular:0.0469-3pmol/ml.Acetylated:0.03125-1pmol/ml |
| Precision | IntraAssayPrecision(Regular):TwohumanurinesamplesweredilutedwithSampleDiluentandruninreplicatesof22inanassay.ThemeanandprecisionofthecalculatedcGMPconcentrationswere:Sample1-0.053pmol/mL,10.6%CVSample2-0.225pmol/mL,11.8%CVIntraAssayPrecision(Acetylated):TwohumanurinesamplesweredilutedwithSampleDiluent,acetylatedandruninreplicatesoftwenty-twoinanassay.ThemeanandprecisionofthecalculatedcGMPconcentrationswere:Sample1-0.032pmol/mL,8.9%CVSample2-0.018pmol/mL,8.3%CVInterAssayPrecision(Regular):TwohumanurinesamplesweredilutedwithSampleDiluentandruninduplicatesinfourteenassaysrunovermultipledaysbyfouroperators.ThemeanandprecisionofthecalculatedcGMPconcentrationswere:Sample1-0.213pmol/mL,14.9%CVSample2-0.083pmol/mL,18.0%CVInterAssayPrecision(Acetylated):TwohumanurinesampleweresdilutedwithSampleDiluent,acetylatedandruninduplicatesineightassaysrunovermultipledaysbyfouroperators.ThemeanandprecisionofthecalculatedcGMPconcentrationswere:Sample1-0.07pmol/mL,15.1%CVSample2-0.04pmol/mL,15.1%CV |
| NumberofSamples | 39samplesinduplicate |
| OtherResources | KitBooklet,MSDS,SalivaSampleHandlingInstructions |
Properties
| StorageTemperature | 4ºC | ||||||||||||||||||||||||||||||||||||||||||
| ShippingTemperature | BlueIce | ||||||||||||||||||||||||||||||||||||||||||
| ProductType | CLIAKits | ||||||||||||||||||||||||||||||||||||||||||
| AssayOverview | TheCyclicGMP(cGMP)CLIA(High-Sensitivity)kitisdesignedtoquantitativelymeasurecGMPpresentinlysedcells,EDTAplasma,urine,salivaandtissueculturemediasamples.ForsampleswherethelevelsofcGMPareexpectedtoberelativelyhigh,theregularformatfortheassaycanbeused.ForsampleswithexpectedlowlevelsofcGMP,anoptionalacetylationprotocolcanbeused.ThekitisuniqueinthatallsamplesandstandardsaredilutedintoanacidicSampleDiluent,whichcontainsspecialadditivesandstABIlizers,forcGMPmeasurement.Thisallowsplasma,urineandsalivasamplestobereadinanidenticalmannertolysedcells.AcidifiedsamplesofcGMParestableandendogenousphosphodiesterasesareinactivatedintheSampleDiluent.AcGMPstandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.AwhitemicrotiterplatecoatedwithanantibodytocapturemouseIgGisprovidedandaneutralizingPlatePrimersolutionisaddedtoalltheusedwells.Standardsordilutedsamples,eitherwithorwithoutacetylation,arePipettedintotheprimedwells.AcGMP-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.ThebindingreactionisinitiatedbytheadditionofamousemonoclonalantibodytocGMPtoeachwell.Afteranovernightincubationat4°C,theplateiswashedandthechemiluminescentsubstrateisadded.ThesubstratereactswiththeboundcGMP-peroxidaseconjugatetoproducelightThegeneratedlightisdetectedinamicrotiterplatereadercapableofreADIngluminescence.TheconcentrationofthecGMPinthesampleiscalculated,aftermakingsuitablecorrectionforthedilutionofthesample,usingsoftwareavailablewithmostplatereaders. | ||||||||||||||||||||||||||||||||||||||||||
| KitOverview |
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| CiteThisProduct | CyclicGMPCLIAKit(High-Sensitivity)(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-210) |
BIOLOGicalDescription
| AlternativeNames | cGMP;3",5"-cyclicGMPCLIAKit,GuanosinecyclicmonophosphateCLIAKit,Cyclic3",5"-GMP;Guanosine3",5"-cyclicphosphateCLIAKit |
| ResearchAreas | CellSignaling,Neuroscience |
| ScientificBackground | Guanosine3’,5’-cyclicmonophosphate(cyclicGMP;cGMP)isacriticalandmultifunctionalsecondmessengerpresentatlevelstypically10-100foldlowerthancAMPinmosttissues.IntracellularcGMPisformedbytheactionoftheenzymeguanylatecyclaseonGTPanddegradedthroughphosphodiesterasehydrolysis(1-3).Guanylatecyclases(GC)areeithersolubleormembranebound(3,4).SolubleGCsarenitricoxideresponsive,whereasmembraneboundGCsrespondtohormonessuchasacetylcholine,insulinandoxytocin.OtherchemicalslikeSEROtoninandhistaminealsocauseanincreaseincGMPlevels(5,6).CyclicGMPregulatescellularcompositionthroughcGMP-dependentkinase,cGMP-dependentionchannelsortransporters,andthroughitshydrolyticdegradationbyphosphodiesterase(1,7) |
| References | 1.Domek-Lopacinska,K.andStrosznajder,JB.(2005)JPhysiolPharmacol56Suppl2,15-34. 2.Lucas,K.A.etal.(2000)PharmacolRev52:375-414. 3.Ashman,DF.,etal.(1963),BiochemBiophysResComm,11:330-4. 4.PotterLR,Abbey-HoschS,andDickeyDM.(2006)EndocrRev27:47-72. 5.Waldman,SAandMurad,F.(1987)PharmacolRevs,39:163-197. 6.TremblayJ,GerzerR,andHametP.(1988),Adv.2ndMessanger&PhosphoproteinRes.,22:319-383. 7.MatsumotoT,KobayashiT,andKamataK.(2003)JSmoothMuscleRes39:67-86. |
ProductImages
RegularFormatTypicalStandardCurvefortheCyclicGMPCLIAKit(High-Sensitivity)(ChemiluminescentImmunoassay)StressXpress®–SKT-210.AssayType:DirectCLIA.DetectionMethod:ChemiluminescentAssay.AssayRange:Regular:0.0469–3pmol/ml.Acetylated:0.03125–1pmol/ml.
AcetylatedFormatTypicalStandardCurvefortheCyclicGMPCLIAKit(High-Sensitivity)(ChemiluminescentImmunoassay)StressXpress®–SKT-210.AssayType:DirectCLIA.DetectionMethod:ChemiluminescentAssay.AssayRange:Regular:0.0469–3pmol/ml.Acetylated:0.03125–1pmol/ml.
Linearitywasdeterminedbytakingtwohumanurinesamples,onewithalowcGMPlevelof0.3pmol/mLandonewithahigherlevelof9.7pmol/mL,andmixingtheminratios.Themeasuredconcentrationswerecomparedtotheexpectedvaluesbasedontheratiosused.
ChemicalstructureofCyclicGMP(Cyclicguanosinemonophosphate)
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