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StressMarq/StressXpress® Cyclic AMP EIA Kit/SKT-209-96/96-well

价格
¥7700.00
货号:SKT-209-96
浏览量:127
品牌:StressMarq
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商品描述

Overview:

ProductNameCyclicAMPEIAKit
Description

QuantitativecolorimetricdetectionofcAMP

SpeciesReactivitySpeciesIndependent
PlatformMicroplate
SampleTypesCelllysates,EDTAPlasma,HeparinPlasma,Saliva,Tissue,TissueCultureMedia,Urine
DetectionMethodColorimetricAssay
AssayTypeDirectEIA(EnzymeImmunoassay)
UtilityEIAkitusedtoquantitativelymeasurecAMPpresentinsamples.
Sensitivity0.64pmol/ml
AssayRangeRegular:0.617-150pmol/ml.Acetylated:0.156-10pmol/ml
PrecisionIntraAssayPrecision(Regular):ThreehumanurinesamplesweredilutedwithSampleDiluentandruninreplicatesof20inanassay.ThemeanandprecisionofthecalculatedcAMPconcentrationswere:Sample1-56.9pmol/mL,8.6%CVSample2-11.9pmol/mL,11.3%CVSample3-6.7pmol/mL,12.3%CVIntraAssayPrecision(Acetylated):TwohumanplasmasamplesweredilutedwithSampleDiluent,acetylatedandruninreplicatesof20inanassay.ThemeanandprecisionofthecalculatedcAMPconcentrationswere:Sample1-1.74pmol/mL,10.4%CVSample2-0.60pmol/mL,11.8%CVInterAssayPrecision(Regular):ThreehumanurinesamplesweredilutedwithSampleDiluentandruninduplicatesintwelveassaysrunovermultipledaysbyfouroperators.ThemeanandprecisionofthecalculatedcAMPconcentrationswere:Sample1-57.1pmol/mL,10.0%CVSample2-10.9pmol/mL,11.5%CVSample3-6.3pmol/mL,11.3%CVInterAssayPrecision(Acetylated):OnehumanurineandtwohumanplasmasampleweredilutedwithSampleDiluent,acetylatedandruninduplicatesintwelveassaysrunovermultipledaysbyfouroperators.ThemeanandprecisionofthecalculatedcAMPconcentrationswere:Sample1-5.22pmol/mL,9.8%CVSample2-0.99pmol/mL,8.1%CVSample3-0.73pmol/mL,15.4%CV
IncubationTime2.5Hours
NumberofSamples39samplesinduplicate
OtherResourcesKitBooklet,MSDS,SalivaSampleHandlingInstructions

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeEIAKits
AssayOverviewTheCyclicAMPEIAkitisdesignedtoquantitativelymeasurecAMPpresentinlysedcells,EDTAandheparinplasma,urine,salivaandtissueculturemediasamples.Fortissuesamples,salivaandurine,wherethelevelsofcAMPareexpectedtoberelativelyhigh,theregularformatfortheassaycanbeused.Forplasmasamplesandsomedilutecelllysatesanoptionalacetylationprotocolcanbeused.ThekitisuniqueinthatallsamplesandstandardsaredilutedintoanacidicSampleDiluent,whichcontainsspecialadditivesandstABIlizers,forcAMPmeasurement.Thisallowsplasma,urineandsalivasamplestobereadinanidenticalmannertolysedcells.AcidifiedsamplesofcAMParestableandendogenousphosphodiesterasesareinactivatedintheSampleDiluent.AcAMPstandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.AclearmicrotiterplatecoatedwithanantibodytocapturesheepIgGisprovidedandaneutralizingPlatePrimersolutionisaddedtoalltheusedwells.Standardsordilutedsamples,eitherwithorwithoutacetylation,arePipettedintotheprimedwells.AcAMP-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.ThebindingreactionisinitiatedbytheadditionofasheepantibodytocAMPtoeachwell.Aftera2hourincubation,theplateiswashedandsubstrateisadded.ThesubstratereactswiththeboundcAMP-peroxidaseconjugate.Afterashortincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength.TheconcentrationofthecAMPinthesampleiscalculated,aftermakingsuitablecorrectionforthedilutionofthesample,usingsoftwareavailablewithmostplatereaders.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-209ACoatedClear96WellPlates1Each
SKC-209BCyclicAMPStandard125µl
SKC-209CStressXpress®CyclicAMPAntibody3ml
SKC-209DStressXpress®CyclicAMPConjugate3ml
SKC-209ESampleDiluent28ml
SKC-209FPlatePrimer5ml
SKC-209GAceticAnhydride2ml
SKC-209HTriethylamine4ml
SKC-209IWashBufferConcentrate30ml
SKC-209JTMBSubstrate11ml
SKC-208KStopSolution5ml
SKC-208LPlateSealer1Each
CiteThisProductCyclicAMPEIAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-209)

BIOLOGicalDescription

AlternativeNames3"-5"-cyclicadenosinemonophosphateEIAKit
ResearchAreasCellSignaling,Neuroscience
ScientificBackgroundAdenosine-3’,5’-cyclicmonophosphate(cyclicAMP)isasecondmessengerthatplaysanimportantroleinintracellularregulation.Specifically,itfunctionsasamediatorofactivityofseveralkeyhormonesincludingepinephrine,glucagon,andACTH(1-4).AdenylatecyclaseisactivatedbythehormonesglucagonandadrenalineandbyGprotein.Liveradenylatecyclaserespondsmorestronglytoglucagon,andmuscleadenylatecyclaserespondsmorestronglytoadrenaline.cAMPdecompositionintoAMPiscatalyzedbytheenzymephosphodiesterase.IntheHumanMetabolomeDatabasethereare166metabolicenzymeslistedthatconvertcAMP(5).OtherbiologicalactionsofcAMPincluderegulationofinnateimmunefunctioning(6),axonregeneration(7),cancer(8),andinflammation(9).
References1.Sutherland,E.W.andRall,T.W.FractionationandCharacterizationofaCyclicAdenineRibonucleotideFormedbyTissueParticles.J.Biol.Chem.,232:1077,1958.
2.Marsh,J.M.Biol.Reprod.,14:30-53,1976.
3.Korenman,S.G.andKrall,J.F.Biol.Reprod.,16:1-17,1977.
4.Kelley,D.J.,Bhattacharyya,A.,Lahvis,G.P.,Yin,J.C.P.,Malter,J.,andDavidson,R.J.Neurosci.Biobehav.Rev.,32(8):1533-1543,2008.
5.http://www.hmdb.ca/metabolites/HMDB00058
6.Serezani,C.H.,Ballinger,M.N.,Aronoff,D.M.,andPeters-Golden,M.Am.J.Resp.CellandMol.Biol.,39(2):127,2008.
7.Hannila,S.S.,andFilbin,M.T.Exp.Neurol.,209(2):321–332,2008.
8.Shankar,D.B,Cheng,J.C.,andSakamoto,K.M.Cancer,104(9):1819-24,2005.
9.GaleaE.andFeinstein,D.L.FASEBJ.,13:2125-2137,1999.

ProductImages

<p>Regular Format Typical Standard Curve for the Cyclic AMP EIA Kit (Enzyme Immunoassay) StressXpress® – SKT-209. Assay Type: Direct EIA. Detection Method: Colorimetric Assay. Assay Range: Regular: 0.617 – 150 pmol/ml. Acetylated: 0.156 – 10 pmol/ml.</p>

RegularFormatTypicalStandardCurvefortheCyclicAMPEIAKit(EnzymeImmunoassay)StressXpress®–SKT-209.AssayType:DirectEIA.DetectionMethod:ColorimetricAssay.AssayRange:Regular:0.617–150pmol/ml.Acetylated:0.156–10pmol/ml.

<p>Acetylated Format Typical Standard Curve for the Cyclic AMP EIA Kit (Enzyme Immunoassay) StressXpress® – SKT-209. Assay Type: Direct EIA. Detection Method: Colorimetric Assay. Assay Range: Regular: 0.617 – 150 pmol/ml. Acetylated: 0.156 – 10 pmol/ml.</p>

AcetylatedFormatTypicalStandardCurvefortheCyclicAMPEIAKit(EnzymeImmunoassay)StressXpress®–SKT-209.AssayType:DirectEIA.DetectionMethod:ColorimetricAssay.AssayRange:Regular:0.617–150pmol/ml.Acetylated:0.156–10pmol/ml.

<p>Chemical structure of Cyclic AMP (Adenosine-3’,5’-cyclic monophosphate)</p>

ChemicalstructureofCyclicAMP(Adenosine-3’,5’-cyclicmonophosphate)

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