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蚂蚁淘在线 / 品牌中心 / StressMarq / StressMarq/StressXpress® Creatinine Serum Detection Kit/SKT-217-192/2-x-96-well
商品描述
Overview:
| ProductName | CreatinineSerumDetectionKit |
| Description | Colorimetricmeasurementofcreatinine |
| SpeciesReactivity | Human,Mouse,Rat,Rabbit,Sheep |
| Platform | Microplate |
| SampleTypes | Plasma,Serum |
| DetectionMethod | ColorimetricAssay |
| AssayType | DirectQuantitativeAssay |
| Utility | Colorimetricassayusedtomeasurecreatinineinsamples. |
| Sensitivity | 0.081mg/dl |
| AssayRange | 0.5-4mg/dl |
| Precision | IntraAssayPrecision:Threehumanserumsampleswereruninreplicatesof20inanassay.Themeanandprecisionofthecalculatedcreatinineconcentrationswere:Sample1-0.99mg/dL,7.9%CVSample2-1.50mg/dL,6.3%CVSample3-3.82mg/dL,4.5%CVInterAssayPrecision:Threehumanserumsampleswereruninduplicatesin19assaysrunovertwoyearsbyfouroperators.Themeanandprecisionofthecalculatedcreatinineconcentrationswere:Sample1-0.91mg/dL,9.6%CVSample2-1.26mg/dL,7.3%CVSample3-3.51mg/dL,8.0%CV |
| NumberofSamples | 91samplesinduplicate |
| OtherResources | KitBooklet,MSDS |
Properties
| StorageTemperature | 4ºC | |||||||||||||||
| ShippingTemperature | BlueIce | |||||||||||||||
| ProductType | DetectionKits | |||||||||||||||
| AssayOverview | TheCreatinineSerumDetectionKitisdesignedtoquantitativelymeasurecreatininepresentinserumsamples.Acreatininestandard,calibratedtoaNISTcreatininestandard,isprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.StandardsorsamplesarePipettedintoaclearmicrotiterplate.Anassaydiluentisaddedtoallstandards,controlsandsamples.ThecolorgeneratingreactionisinitiatedwiththeStressXpress®CreatinineReagent,whichispipettedintoeachwell.Theassayutilizesakineticabsorbancemethodtoovercomeinterferencebycoloredcompoundsinserum.Theabsorbanceofthecoloredproductisreadafter1minuteinamicrotiterplatereadercapableofmeasuring490nmwavelength.At30minutestheopticaldensityisreadagain.TheconcentrationofcreatinineiscalculatedusingthedeltaoftheopticaldensityreADIngsat30and1minutecomparedtothecurvegeneratedfromthestandards,orbyusingtheExcelworksheetavailableforfreedownloadatourwebsite.TheJaffereactionusedinthiskithasbeenmodifiedtoreadcreatininelevelsinserum,8. | |||||||||||||||
| KitOverview |
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| CiteThisProduct | CreatinineSerumDetectionKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-217) |
BIOLOGicalDescription
| AlternativeNames | N-Carbamimidoyl-N-methylglycineDetectionKit,MethylguanidoaceticacidDetectionKit |
| ResearchAreas | CardiovascularSystem,CellSignaling |
| ScientificBackground | Creatinine(2-amino-1-methyl-5H-imadazol-4-one)isametaboliteofphosphocreatine(p-creatine),amoleculeusedasastoreforhigh-energyphosphatethatcanbeutilizedbytissuesfortheproductionofATP(1).Creatineeithercomesfromthedietorsynthesizedfromtheaminoacidsarginine,glycine,andmethionine.Thisoccursinthekidneysandliver,althoughotherorgansystemsmaybeinvolvedandspecies-specificdifferencesmayexist(2).Creatineandp-creatineareconvertednon-enzymaticallytothemetabolitecreatinine,whichdiffusesintothebloodandisexcretedbythekidneys.Invivo,thisconversionappearstobeirreversIBLeandinvitroitisfavoredbyhighertemperaturesandlowerpH2.Creatinineformsspontaneouslyfromp-creatine(3).Undernormalconditions,itsformationoccursataratethatisrelativelyconstantandasintra-individualvariationis<15% from day to day, creatinine is a useful tool for normalizing the levels of other molecules found in urine. Additionally altered creatinine levels may be associated with other conditions that result in decreased renal blood flow such as diabetes and cardiovascular disease (4-6). |
| References | 1.Wallimann,T.etal.,Biochem.J.,2000,281,21-40. 2.Wyss,M.andKaddurah-Daouk,R.,Physiol.Rev.,2000,80,1107-1213. 3.RajaIyengar,M.etal.,J.Biol.Chem,1985,260,7562-7567. 4.Manjunath,G.etal.,Postgrad.Med.2001,110,55-62. 5.Gross,J.L.etal.,DiabetesCare,2005,28,164-176. 6.Anavekar,N.S.etal.,NewEngl.J.Med.,2004,351,1285-1295. |
ProductImages
TypicalStandardCurveforCreatinineSerumDetectionKitStressXpress®–SKT-217.AssayType:DirectEnzyme.DetectionMethod:ColorimetricAssay.AssayRange:0.5–4mg/dl.
Linearitywasdeterminedbytakingtwohumanserumsamples,onewithalowdilutedcreatininelevelof0.75mg/dLandonewithahigherlevelof3.78mg/dLandmixingthemingivenratios.Themeasuredconcentrationswerecomparedtotheexpectedvalues.
Creatineandp-creatineareconvertednon-enzymaticallytothemetabolitecreatinine,whichdiffusesintothebloodandisexcretedbythekidneys.Invivo,thisconversionappearstobeirreversibleandinvitroitisfavoredbyhighertemperaturesandlowerpH2.Creatinineformsspontaneouslyfromp-creatine.
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