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StressMarq/StressXpress® Cortisone CLIA Kit/SKT-206-96/96-well

价格
¥7960.00
货号:SKT-206-96
浏览量:127
品牌:StressMarq
服务
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商品描述

Overview:

ProductNameCortisoneCLIAKit
Description

Chemiluminescentmeasurementofcortisone

SpeciesReactivitySpeciesIndependent
PlatformMicroplate
SampleTypesDriedFecalSamples,Plasma,Saliva,Serum,TissueCultureMedia,Urine
DetectionMethodChemiluminescentAssay
AssayTypeSandwichCLIA(ChemiluminescentImmunoassay)
UtilityCLIAkitusedtoquantitativelymeasurethecortisonepresentinsamples.
Sensitivity10.6pg/ml
AssayRange78.1-20,000pg/ml
PrecisionIntraAssayPrecision:ThreehumansamplesweredilutedwithAssayBufferandruninreplicatesof20inanassay.ThemeanandprecisionofthecalculatedCortisoneconcentrationswere:Sample1-7902.0pg/mL,7.9%CVSample2-596.1pg/mL,5.7%CVSample3-234.0pg/mL,10.1%CVInterAssayPrecision:ThreehumansamplesweredilutedwithAssayBufferandruninduplicatesinthirteenassaysrunovermultipledaysbythreeoperators.ThemeanandprecisionofthecalculatedCortisoneconcentrationswere:Sample1-7904.0pg/mL,10.0%CVSample2-662.7pg/mL,11.1%CVSample3-262.9pg/mL,12.9%CV
IncubationTime2hours
NumberofSamples37samplesinduplicate
OtherResourcesKitBooklet,MSDS,SteroidSolidExtractionProtocol,SalivaSampleHandlingInstructions

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeCLIAKits
AssayOverviewTheCortisoneCLIAkitisdesignedtoquantitativelymeasureCortisonepresentinextracteddriedfecalsamples,urine,saliva,andserumsamples.Thiskitmeasurestotalcortisoneinserumandplasmaandinextractedfecalsamples.Acortisonestandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.StandardsordilutedsamplesarePipettedintoawhitemicrotiterplatecoatedwithanantibodytocapturerabbitantibodies.Acortisone-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.Thebindingreactionisinitiatedbytheadditionofapolyclonalantibodytocortisonetoeachwell.Afteratwohourincubationtheplateiswashedandthechemiluminescentsubstrateisadded.Thesubstratereactswiththeboundcortisone-peroxidaseconjugatetoproducelight.ThegeneratedlightisdetectedinamicrotiterplatereadercapableofreADIngluminescence.Theconcentrationofthecortisoneinthesampleiscalculated,aftermakingsuitablecorrectionforthedilutionofthesample,usingsoftwareavailablewithmostplatereaders.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-206ACoatedClear96WellPlates1Each
SKC-206BCortisoneStandard50µl
SKC-206CStressXpress®CortisoneCLIAAntibody3ml
SKC-206DStressXpress®CortisoneCLIAConjugateConcentrate1ml
SKC-206EConjugateDiluent3ml
SKC-206FAssayBuffer50ml
SKC-206GDissociationReagent1ml
SKC-206HWashBufferConcentrate30ml
SKC-206ISubstrateSolutionA6ml
SKC-206JSubstrateSolutionB6ml
SKC-206KPlateSealer1Each
CiteThisProductCortisoneCLIAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-206)

BIOLOGicalDescription

AlternativeNames(8S,9S,10R,13S,14S,17R)-17-Hydroxy-17-(2-hydroxyacetyl)-10,13-dimethyl-1,2,6,7,8,9,12,14,15,16-decahydrocyclopenta[a]phenanthrene-3,11-dioneCLIAKit
ResearchAreasCellSignaling
ScientificBackgroundCortisone(C21H28O5,Kendall’sCompound‘E’)wasidentifiedbyMason,MyersandKendallin1936asCompoundEextractedfrombovinesuprarenalglandtissuethathadthequalitativebutnotquantitativeactivityofcortin.Thepresenceofmultiplecortin-likecompoundsledtheauthorstospeculatethatthestudyofCompoundEwouldrevealthenatureofcortin(1).CompoundEisnowcalledcortisoneandthemoreactiveCompoundF,cortisol,andtheconcentrationsofthesetwoglucocorticoidsvaryduetotheactivityoftwo11ß-hydroxysteroiddehydrogenases(11-HSD)(2,3).WhilemosttissueshavetheABIlitytoexpresseitherenzyme,11ß-HSD1isfoundprimarilyintheliverwhereitconvertscortisonetocortisolwhile11ß-HSD2isfoundintissuessuchasthekidneywherecortisolreceptorbindingisrequired.11ß-HSD2deactivatescortisoltocortisone,prohibitingreceptoractivation.Thisglucocorticoid“shuttle”helpstoinitiateandregulatetheanti-inflammatoryresponse,makingcortisoneoneofthemodern“wonderdrugs”.onitoringtheratioofcortisone:cortisolhasapplicationsindiabetes,obesity,metabolicsyndrome,osteoporosis,andchronicfatiguesyndromeinadditiontoadrenaldiseases(4-7).Cortisoneandcortisolconcentrationsexhibitapredictablediurnalpatternandcanbemeasuredinextracteddriedfeces,orinserum,plasma,salivaandurine.Arecentpublication(8)hassuggestedthatsalivarycortisoneisagoodsurrogateMarkerforserumcortisol.
References1.Mason,HL,etal.J.Biol.Chem.,1936116:267-276.
2.Mason,HL,et.al.J.Biol.Chem.,1938124:459-474.
3.Hillier,SG.“DiamondsareForever:theCortisoneLegacy”J.Endo.,2007195:1-6.
4.vanRaalte,DH,etal.Eur.J.Clin.Invest.200939(2):81-93.
5.Pierotti,S,etal.J.SteroidBiochem.Mol.Biol.2008108(3-5):292-9.
6.Hadoke,PWF,etal.Br.J.Pharmacol.2009156:689-712.
7.Jerkes,WK,etal.J.PsychosomaticRes.200660:145-153.
8.Perogamvros,I,etal.JClin.Endocrin.Metab.2010August4(Epubaheadofprint).

ProductImages

<p>Typical Standard Curve for the Cortisone CLIA Kit (Chemiluminescent Immunoassay) StressXpress® – SKT-206. Assay Type: Sandwich CLIA. Detection Method: Chemiluminescent Assay. Assay Range: 78.1 – 20,000 pg/ml.</p>

TypicalStandardCurvefortheCortisoneCLIAKit(ChemiluminescentImmunoassay)StressXpress®–SKT-206.AssayType:SandwichCLIA.DetectionMethod:ChemiluminescentAssay.AssayRange:78.1–20,000pg/ml.

<p>Linearity was determined by taking two serum samples treated with Dissociation Reagent and diluted 1:50 with Assay Buffer, one with a low diluted cortisone level of 1,336 pg/mL and one with a higher diluted level of 4,055 pg/mL, and mixing them. The measured concentrations were compared to the expected values based on the ratios used.</p>

LinearitywasdeterminedbytakingtwoserumsamplestreatedwithDissociationReagentanddiluted1:50withAssayBuffer,onewithalowdilutedcortisonelevelof1,336pg/mLandonewithahigherdilutedlevelof4,055pg/mL,andmixingthem.Themeasuredconcentrationswerecomparedtotheexpectedvaluesbasedontheratiosused.

<p>Chemical structure of Cortisone</p>

ChemicalstructureofCortisone

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