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StressMarq/StressXpress® Cortisol EIA Kit/SKT-201-96/96-well

价格
¥5220.00
货号:SKT-201-96
浏览量:127
品牌:StressMarq
服务
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商品描述

Overview:

ProductNameCortisolEIAKit
Description

Quantitativecolorimetricdetectionofcortisol

SpeciesReactivitySpeciesIndependent
PlatformMicroplate
SampleTypesDriedFecalSamples,EDTAPlasma,HeparinPlasma,Saliva,Serum,TissueCultureMedia,Urine
DetectionMethodColorimetricAssay
AssayTypeSandwichEIA(EnzymeImmunoassay)
UtilityEIAkitusedtoquantitativelymeasurecortisolpresentinsamples.
Sensitivity17.3pg/ml
AssayRange100-3200pg/ml
PrecisionIntraAssayPrecision:ThreehumansamplesweredilutedwithAssayBufferandruninreplicatesof20inanassay.ThemeanandprecisionofthecalculatedCortisolconcentrationswere:Sample1-1174.3pg/mL,6%CVSample2-475.9pg/mL,5.6%CVSample3-177.4pg/mL,14.7%CVInterAssayPrecision:ThreehumansamplesweredilutedwithAssayBufferandruninduplicatesintenassaysrunovermultipledaysbyfouroperators.ThemeanandprecisionofthecalculatedCortisolconcentrationswere:Sample1-1188.1pg/mL,7.2%CVSample2-508.7pg/mL,6.3%CVSample3-199.7pg/mL,10.9%CV
IncubationTime90Minutes
NumberofSamples40samplesinduplicate
OtherResourcesKitBooklet,MSDS,SteroidSolidExtractionProtocol,SalivaSampleHandlingInstructions

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeEIAKits
AssayOverviewTheCortisolEIAkitisdesignedtoquantitativelymeasurecortisolpresentindriedfecalextracts,saliva,urine,serum,plasmaandtissueculturemediasamples.Thiskitmeasurestotalcortisolinextractedsamplesandinserumandplasmaandfreecortisolinsalivaandurine.Acortisolstandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.StandardsordilutedsamplesarePipettedintoaclearmicrotiterplatecoatedwithanantibodytocapturemouseantibodies.Acortisol-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.Thebindingreactionisinitiatedbytheadditionofamonoclonalantibodytocortisoltoeachwell.Afteran1hourincubationtheplateiswashedandsubstrateisadded.Thesubstratereactswiththeboundcortisol-peroxidaseconjugate.Afterashortincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength.Theconcentrationofthecortisolinthesampleiscalculated,aftermakingsuitablecorrectionforthedilutionofthesample,usingsoftwareavailablewithmostplatereaders.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-201AClearMicrotitre96wellPlate1Plate
SKC-201BCortisolStandard125µl
SKC-201CStressXpress®CortisolAntibody3ml
SKC-201DStressXpress®CortisolConjugate3ml
SKC-201EAssayBuffer28ml
SKC-201FDissociationReagent1ml
SKC-201GWashBufferConcentrate30ml
SKC-201HTMBSubstrate11ml
SKC-201IStopSolution5ml
SKC-201JPlateSealer1Each
CiteThisProductCortisolEIAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-201)

BIOLOGicalDescription

AlternativeNames(11β)-11,17,21-trihydroxypregn-4-ene-3,20-dioneEIAKit
ResearchAreasCancer,CellSignaling,Neuroscience,OxidativeStress
ScientificBackgroundCortisol,C21H30O5,(hydrocortisone,compoundF)istheprimaryglucocorticoidproducedandsecretedbytheadrenalcortex.Itisoftenreferredtoasthe“stresshormone”asitisinvolvedintheresponsetostressanditaffectsbloodpressure,bloodsugarlevels,andotheractionsofstressadaptation.Immunologically,cortisolfunctionsasanimportantanti-inflammatoryandplaysaroleinhypersensitivity,immunosuppression,anddiseaseresistance(1).Inthemetabolicaspect,cortisolpromotesgluconeogenesis,liverglycogendeposition,andthereductionofglucoseutilization(2).ProductionofcortisolfollowsanACTH-dependentcircADIanrhythm,withapeaklevelinthemorninganddecreasinglevelsthroughouttheday.Mostserumcortisol,allbutabout4%,isboundtoproteinsincludingcorticosteroidbindingglobulinandserumalbumin(1,3).Onlyfreecortisolisavailabletomostreceptorsanditisthroughthesereceptorsthatphysiologicalprocessesaremodulated.Abnormalcortisollevelsarebeingevaluatedforcorrelationwithavarietyofdifferentconditions,suchasprostatecancer(4),depression(5),andschizophrenia(6).ItisalreadyknownthatabnormallevelsofcortisolareinvolvedinCushing’sSyndromeandAddision’sdisease(7).
References1.E.Friess,etal.,EurJClinInvest,2000,30,Suppl3:46-50.
2.Freeman,Scott,2002.BiologicalScience.PrenticeHall;2ndPkgedition(December30,2004).
3.C.Longscope.,J.Endocrinology,1996,,SupplS125-S127.
4.J.Herbert,Lancet,1995345,1193-1194.
5.A.Michael,etal.,Biol.Psychiatry,2000,48,989-95.
6.C.R.DequetandD.J.Wallace,CurrentOpin.Ivest.Drugs,2001,8,1045-53.
7.W.M.Jeffries,Med.Hypotheses,1998,51,114-4.

ProductImages

<p>Typical Standard Curve for the Cortisol EIA Kit (Enzyme Immunoassay) StressXpress® – SKT-201. Assay Type: Sandwich EIA. Detection Method: Colorimetric Assay. Assay Range: 100 – 3200 pg/ml.</p>

TypicalStandardCurvefortheCortisolEIAKit(EnzymeImmunoassay)StressXpress®–SKT-201.AssayType:SandwichEIA.DetectionMethod:ColorimetricAssay.AssayRange:100–3200pg/ml.

<p>Linearity was determined by taking two human urine samples diluted 1:140, one with a low diluted cortisol level of 163.9 pg/mL and one with a higher diluted level of 2,974.9 pg/mL and mixing them in the ratios given below. The measured concentrations were compared to the expected values based on the ratios used.</p>

Linearitywasdeterminedbytakingtwohumanurinesamplesdiluted1:140,onewithalowdilutedcortisollevelof163.9pg/mLandonewithahigherdilutedlevelof2,974.9pg/mLandmixingthemintheratiosgivenbelow.Themeasuredconcentrationswerecomparedtotheexpectedvaluesbasedontheratiosused.

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