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StressMarq/StressXpress® Catalase Activity Kit/SKT-215-192/2-x-96-well

价格
¥7700.00
货号:SKT-215-192
浏览量:116
品牌:StressMarq
服务
全国联保
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商品描述

Overview:

ProductNameCatalaseActivityKit
Description

Quantitativecolorimetricmeasurementofcatalaseactivity

SpeciesReactivitySpeciesIndependent
PlatformMicroplate
SampleTypesCelllysates,EDTAPlasma,Erythrocytes,HeparinPlasma,Serum,Tissue
DetectionMethodColorimetricAssay
AssayTypeDirectEnzymeActivityAssay
UtilityColorimetricassayusedtoquantitativelymeasurecatalaseactivityinavarietyofsamples.
Sensitivity0.052U/ml
AssayRange0.156-5U/ml
PrecisionIntraAssayPrecision:ThreehumanserumsamplesdilutedinAssayBufferwereruninreplicatesof20inanassay.Themeanandprecisionofthecalculatedconcentrationswere:Sample1-1.71U/mL,3.5%CVSample2-0.84U/mL,4.0%CVSample3-0.48U/mL,4.8%CVInterAssayPrecision:ThreehumanserumsamplesdilutedinAssayBufferwereruninduplicatesintwenty-oneassaysrunovermultipledaysbythreeoperators.Themeanandprecisionofthecalculatedconcentrationswere:Sample1-1.79U/mL,11.9%CVSample2-0.94U/mL,9.8%CVSample3-0.53U/mL,12.3%CV
IncubationTime45Minutes
NumberofSamples89samplesinduplicate
OtherResourcesKitBooklet,MSDS

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeActivityKits
AssayOverviewTheCatalaseActivityKitisdesignedtoquantitativelymeasurecatalaseactivityinavarietyofsamples.Abovinecatalasestandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffofthestandardcurve.SamplesaredilutedintheprovidedAssayBufferandaddedtothewellsofahalfareaclearplate.Hydrogenperoxideisaddedtoeachwellandtheplateincubatedatroomtemperaturefor30minutes.ThesuppliedColorimetricDetectionReagentisadded,followedbydilutedhorserADIshperoxidaseandincubatedatroomtemperaturefor15minutes.TheHRPreactswiththesubstrateinthepresenceofhydrogenperoxidetoconvertthecolorlesssubstrateintoapink-coloredproduct.Thecoloredproductisreadat560nm.IncreasinglevelsofcatalaseinthesamplescausesadecreaseinH2O2concentrationandareductioninpinkproduct.Theactivityofthecatalaseinthesampleiscalculatedaftermakingasuitablecorrectionforanydilution,usingsoftwareavailablewithmostplatereaders.TheresultsareexpressedintermsofunitsofcatalaseactivitypermL.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-215AClear96wellHalfAreaPlates2Plates
SKC-215BCatalaseStandard90µl
SKC-215CAssayBufferConcentrate25ml
SKC-215DHydrogenPeroxideReagent5ml
SKC-215EColorimetricDetectionReagent5ml
SKC-215FHorseradishPeroxidaseConcentrate120µl
CiteThisProductCatalaseActivityKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-215)

BIOLOGicalDescription

AlternativeNamesCas1ActivityKit,CATActivityKit,Cs1ActivityKit,MGC138422ActivityKit,MGC138424ActivityKit
ResearchAreasCancer,Apoptosis,OxidativeStress
ScientificBackgroundHydrogenperoxide,H2O2isoneofthemostfrequentlyoccurringreactiveoxygenspecies.Itisformedeitherintheenvironmentorasaby-productofaerobicmetabolism,superoxideformationanddismutation,orasaproductofoxidaseactivity.Bothexcessivehydrogenperoxideanditsdecompositionproducthydroxylradical,formedinaFenton-typereaction,areharmfulformostcellcomponents.Itsrapidremovalisessentialforallaerobicallylivingprokaryoticandeukaryoticcells(1,2).Hydrogenperoxidehowevercanactasasecondmessengerinsignaltransductionpathways,inimmunecellactivation,inflammationprocesses,cellproliferation,andapoptosis(3-5).Oneofthemostefficientwaysofremovingperoxideisthroughtheenzymecatalase,whichisencodedbyasinglegene,andishighlyconservedamongspecies(6-8).Mammals,includinghumansandmice,expresscatalaseinalltissues,andahighconcentrationofcatalasecanbefoundintheliver,kidneysanderythrocytes(9,10).Theexpressionisregulatedattranscription,post-transcriptionandpost-translationlevels6,(11).Highcatalaseactivityisdetectedinperoxisomes(12).Morerecently,shortwavelengthUVradiationhasbeenshowntoproducereactiveoxygenspecies(ROS)throughtheactionofcatalase(13).ThisresponseisthoughttoactasamechanismtoprotectDNAbyconvertingdamagingUVradiationintoROSspeciesthatcanbemetabolizedanddetoxifiedbycellularantioxidantenzymes.
References1.CABIscolE,TamaritJ,RosJ.(2000)Int.Microbiol.3:3–8.
2.KirkmanHN,GaetaniGF.(2007)TrendsBiochemSci.32:44–50.
3.PeusD,etal.FreeRadic.(1999)Biol.Med.27:1197–1202.
4.RahmanI,andAdcockIM.(2006)Eur.Respir.J.28:219–242.
5.VealEA,DayAM,MorganBA.(2007)Mol.Cell.26:1–14.
6.Reimer,DL,Bailley,J,Singh,SM.(1994)Genomics.21:325–336.
7.Quan,.Korneluk,RGTropak,MB,Gravel,RA.(1986)NucleicAcidsRes.14:5321–5335.
8.Nakashima,H.,etal.(1989)Gene.79:279–288.
9.DeisSEROth,A,Dounce,AL.(1970)Physiol.Rev.50:319–375.
10.Schisler,NJ,Singh,SM.(1987)Genome.29:748–760.
11.Masters,C,Pegg,M,Crane,D.(1986)Mol.CellBiochem.70:113–120.
12.Chance,B,Sies,H,Boveris,A.(1979)Physiol.Rev.59:527–605.
13.Heck,DE,Vetrano,AM,Mariano,TM&Laskin,JD.(2003)J.Biol.Chem.278:22432–22436.

ProductImages

<p>Typical Standard Curve for Catalase Activity Kit (Enzyme Activity Assay) StressXpress® – SKT-215. Assay Type: Direct Enzyme. Detection Method: Colorimetric Assay. Assay Range: 0.156 – 5 U/ml.</p>

TypicalStandardCurveforCatalaseActivityKit(EnzymeActivityAssay)StressXpress®–SKT-215.AssayType:DirectEnzyme.DetectionMethod:ColorimetricAssay.AssayRange:0.156–5U/ml.

<p>Linearity was determined by taking two serum samples, one with a high known catalase activity of 1.163 U/mL and the other with a lower catalase activity of 0.485 U/mL and mixing them in given ratios. The measured activities were compared to the expected values based on the ratios used.</p>

Linearitywasdeterminedbytakingtwoserumsamples,onewithahighknowncatalaseactivityof1.163U/mLandtheotherwithalowercatalaseactivityof0.485U/mLandmixingthemingivenratios.Themeasuredactivitieswerecomparedtotheexpectedvaluesbasedontheratiosused.

<p>Catalase catalyzes the breakdown of Hydrogen Peroxide.</p>

CatalasecatalyzesthebreakdownofHydrogenPeroxide.

ProductCitations(1)

OtherCitations

MarkersofoxidativestressinumbilicalcordbloodfromG6PDdeficientAfricannewborns.

Stadem,P.S.etal.(2017)PLoSOne.12(2):e0172980.

PubMedID:28235023ReactivityHumanApplications:Umbilicalcordblood

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