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StressMarq/StressXpress® Alpha B Crystallin ELISA Kit/SKT-123-96/96-well

价格
¥12880.00
货号:SKT-123-96
浏览量:127
品牌:StressMarq
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商品描述

Overview:

ProductNameAlphaBCrystallinELISAKit
Description

ColorimetricdetectionofAlphaBCrystallin

SpeciesReactivityHuman
PlatformMicroplate
SampleTypesCelllysates,Serum,Tissue
DetectionMethodColorimetricAssay
AssayTypeSandwichELISA(Enzyme-linkedImmunosorbentAssay)
UtilityELISAkitusedtoquantitatealphaBcrystallinconcentrationinsamples.
Sensitivity0.009ng/ml
AssayRange0.352-22.5ng/ml
IncubationTime30minutes
NumberofSamples40samplesinduplicate
OtherResourcesKitBooklet,MSDS

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeELISAKits
AssayOverview1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add100µLofStandardorsampletoappropriatewells.3.CoverplatewithPlateSealerandincubateatroomtemperature(20-25°C)for1hour.4.Washplatefourtimeswith1XWashBuffer.5.Add100µLofBiotinylatedAntibodyWorkingSolutiontoeachwell.6.CoverplatewithPlateSealerandincubateatroomtemperaturefor1hour.7.Washplatefourtimeswith1XWashBuffer.8.Add100µLofStreptavidin-HRPWorkingSolutiontoeachwell.9.CoverplatewithPlateSealerandincubateatroomtemperaturefor30minutes.10.Washplatefourtimeswith1XWashBuffer.11.Add100µLofTMBSubstratetoeachwell.12.Developtheplateinthedarkatroomtemperaturefor30minutes.13.Stopreactionbyadding100µLofStopSolutiontoeachwell.14.Measureabsorbanceonaplatereaderat450nm.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-123AAnti-AlphaBCrystallinImmunoassayPlate1Plate
SKC-123B5XAlphaBCrystallinExtractionReagent1vial/10ml
SKC-123CRecombinantAlphaBCrystallinStandard2vials
SKC-123DStandardandSampleDiluent1vial/50ml
SKC-123E10XWashBufferConcentrate1vial/100ml
SKC-123FAnti-AlphaBCrystallinBiotinylatedAntibodyConcentrate1vial/150µl
SKC-123GAnti-AlphaBCrystallinBiotinylatedAntibodyDiluent1vial/13ml
SKC-123HStreptavidin:HRPConcentrate1vial/150µl
SKC-123IStreptavidin:HRPDiluent1vial/13ml
SKC-123JTMBSubstrate1vial/13ml
SKC-123KStopSolution1vial/13ml
CiteThisProductAlphaBCrystallinELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-123)

BIOLOGicalDescription

AlternativeNamesAACRYAELISAKit,CRYA2ELISAKit,CRYABELISAKit,CTPP2ELISAKit,HSPB5ELISAKit,NYRen27antigenELISAKit,alphacrystallinBELISAKit
ResearchAreasCancer,HeatShock
ScientificBackgroundThealpha-crystallinsaremajorwater-solublelensstructuralproteinsofthevertebrateeyethatarerelatedtothesmallheatshockproteinfamily.Thealpha-crystallinspossessstructuralandfunctionalsimilaritieswithHSP25andHSP27(1).Mammalianlenscystallinsaredividedintoalpha,betaandgammafamilies.alphaandbetafamiliesarefurtherdividedintoacidicandbasicgroups(alpha-Aandalpha-Brespectively).Inthelens,alpha-crystallinprimarilyfunctionstomaintainproperrefractiveindex,howeveritcanalsofunctionasamolecularchaperonethatbindstothedenaturedproteins,keepingtheminsolutionandtherebymaintainingthetranslucencyofthelens.Whencellularstressoccurs,alpha-crystallinentersits’phosphorylatedstateandmayserveastructuralcontrolfunctionandplayaroleinproteinmaintenance(2).Inadditiontotheirinteractionwithproteins,alpha-crystallinsalsointeractwithnativemoleculessuchasmembraneproteins,Golgimatrixprotein,structuralproteins,nuclearproteinsandDNA(3,4,5,6,and7).Twootherfunctionsareanautokinaseactivityandparticipationintheintracellulararchitecture,andithasalsobeenproventhatbothalpha-AandBpreventapoptosisbyinhibitingcaspases(8).Specifically,alpha-Bcystallinisfoundinmanycellsandorgansoutsidethelens,andalphaBisoverexpressedinseveralneurologicaldisordersandincelllinesunderstressconditions(9).
References1.MerckK.B.etal.(1993)JBiolChem.268:1046-1052.
2.HorwitzJ.(1992)ProcNatlAcadSciUSA89(21):10449-10453.
3.CobbB.A.andPetrashJ.M.(2002)Biochemistry.41:483-490
4.HorwitzJ.(2003)ExpEyeRes.76:145-153.
5.BullardB.etal.(2004)JBiolChem.279:7917-7924.
6.GangalumR.K.,SchIBLerM.J.andBhatS.P.(2004)JBiolChem.279:43374.43377.
7.MaddalaR.andRaoV.P.(2005)ExpCellRes.306:203-215.
8.YaungJ.,etal.(2007)MolecularVision13:566-577.
9.HeadM.W.etal.(2000)NeuropatholApplNeurobiol.26:304-312.

ProductImages

<p>Typical Standard Curve for the Alpha B Crystallin ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-123. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.352 – 22.5 ng/mL.</p>

TypicalStandardCurvefortheAlphaBCrystallinELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-123.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:0.352–22.5ng/mL.

ProductCitations(1)

OtherCitations

Levelofvitreousalpha-Bcrystallinineyeswithrhegmatogenousretinaldetachment.

Baba,T.,Oshitari,T.andYamamoto,S.(2014)GraefesArchClinExpOphthalmol.253(8):1251-4.

PubMedID:25311653ReactivityHumanApplications:Vitreoussamples

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