Clone/AB feature:

(#6S34)

Antibody generation:

This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse recombinant protein of Frizzled 2.

Antigen:

recombinant mouse protein Frizzled 3

Stability:

Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.

Reconstitution:

Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

Description:

Wnt signaling is involved in variety of developmental processes including cell fate determination, cell polarity, tissue patterning and control of cell proliferation. Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway. The founding member of this family was identified in Drosophila based on its role in tissue polarity in the adult cuticle and named for the disorganized appearance of bristle hairs on the mutant. Ten mammalian Frizzled genes have been identified to date. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent Nterminal signal peptide, a highly conserved extracellular cysteinerich domain, a variablelength linker region, a sevenpass transmembrane domain, and a variablelength C-terminal tail. One of the most conserved regions of the Frizzled proteins is the extracellular cysteine-rich domain (CRD) which spans approximately 120 AA and contains 10 invariant cysteines. Mouse Frizzled-2 shows 100% amino acid identity to human and rat Frizzled2 in the CRD region. Frizzled2 expression is greater in embryonic than adult tissues, with heart, brain, lung, kidney and gut showing the highest levels. Relia Tech类似于我们在大肠杆菌中的蛋白质表达服务，我们还提供了围绕昆虫细胞中基因和蛋白质表达（尤其是信号蛋白质）的模块化服务系统。ReliaTech的“杆状病毒表达服务（BVS）”分为一系列步骤，这些步骤可以单独执行或组合执行，因此您可以选择实际需要的步骤。这些服务旨在充分满足从基因表达到蛋白质纯化的所有特定需求-从通过重组杆状病毒构建，高滴度重组杆状病毒生产，重组蛋白批量生产以及最终蛋白质纯化的基因克隆到杆状病毒转移载体开始。所有步骤均在无血清培养基中进行，以确保安全性并产生高纯度的重组蛋白。除常规项目更新外，您还将在每个服务步骤的最后收到进度报告。我们将根据您的授权逐步指导您继续进行。