StABIlity:

ThelyophilizedhumanFGF-9,thoughstableatroomtemperature,isbeststoreddesiccatedbelow0°C.ReconstitutedhumanFGF-9shouldbestoredat-20°Cto-70°C.Repeatedfreeze/thawcycleswillresultinsignificantlossofactivity.

Reconstitution:

Forreconstitutionitisrecommendedtoaddaceticacid(50mM)containingatleast0.1%BSA/HSA(orFCS)toprepareastocksolutionofnolessthan100mg/ml.Fordilutionitisrecommendedtousesterilephosphate-bufferedsalinecontainingatleast0.1%BSA/HSAorusingmediumwithFCSfordilution.

Description:

TheFGFfamilyiscomposedofatleast22polypeptidesthatshowavarietyofBIOLOGicalactivitiestowardscellsofmesenchymal,neuronalandepithelialorigin.Allmembersareheparin-bindinggrowthfactors.AllFGFshavetwoconservedcysteineresiduesandshare30-50%sequenceidentityattheaminoacidlevel.FGF-9,alsonamedglia-activatingfactor,wasoriginallyidentifiedandpurifiedfromthesupernatantofahumangliomacelllineasamitogenforglialcells.FGF-9hasalsobeenshowntostimulatetheproliferationofoligodendrocytestype2astrocyteProgenitorcells,PC12cellsandBalb/c3T3cells.UnlikeacidicandbasicFGF,theFGF-9moleculeisnotamitogenforhumanvascularendothelialcells.FromthefourFGFreceptorsknown,FGF-9bindsonlytotheFGF-R3.ConstitutiveactiveFGF-R3inhumanisconnectedwithanormalbonedevelopment.ThehumanFGF-9CDNAencodesa208aminoacidresidueproteinthatcontainsasingle,potentialN-linkedglycosylationsite.Thenativeproteinisglycosylatedandisefficientlysecretedaftersynthesis,althoughFGF-9lacksatypicalsecretionsignal.RatandmouseFGF-9showaveryhighhomologytohumanFGF-9.ThetranscriptsforFGF-9havebeenfoundinbrainandinkidneytissue. Relia Tech类似于我们在大肠杆菌中的蛋白质表达服务，我们还提供了围绕昆虫细胞中基因和蛋白质表达（尤其是信号蛋白质）的模块化服务系统。ReliaTech的“杆状病毒表达服务（BVS）”分为一系列步骤，这些步骤可以单独执行或组合执行，因此您可以选择实际需要的步骤。这些服务旨在充分满足从基因表达到蛋白质纯化的所有特定需求-从通过重组杆状病毒构建，高滴度重组杆状病毒生产，重组蛋白批量生产以及最终蛋白质纯化的基因克隆到杆状病毒转移载体开始。所有步骤均在无血清培养基中进行，以确保安全性并产生高纯度的重组蛋白。除常规项目更新外，您还将在每个服务步骤的最后收到进度报告。我们将根据您的授权逐步指导您继续进行。