RecombinantCXCL12a(SDF-1α)

ORDERINGINFORMATIONRELATEDPRODUCTSORDERINGINFORMATIONRELATEDPRODUCTS

CatalogNo.Size#1009Anti-CXCR4N-terminusAntibody

50180P-22ug#1012Anti-CXCR42ndExtracellularLoop

50180P-1010ugAntibody50180P-5050ug

50180P-100100ug

50180P-10001000ug

BACKGROUNDBACKGROUND

CXCL12aandCXCL12b,alsoknownasStromalCell-DerivedFactor1αand1β(SDF-1αand

SDF-1β),aresmallcytokinesthatbelongtotheintercrinefamily.BothformsofCXCL12areproducedbyalternatesplicingofthesamegene.CXCL12isstronglychemotacticforT-lymphocytesandmonocytes,anditplaysanimportantroleinangiogenesisbyrecruitingendothelialProgenitorcells(EPCs)fromthebonemarrowthroughaCXCR4dependentmechanism.ItisthisfunctionofCXCL12thatmakesitaveryimportantfactorincarcinogenesisandtheneovascularisationlinkedtotumorprogression.CXCL12alsohasaroleintumormetastasiswherecancercellsthatexpressthereceptorCXCR4areattractedtometastasistargettissuesthatreleasetheligand,CXCL12.Inbreastcancer,however,increasedexpressionofCXCL12isassociatedwithareducedriskofdistantmetastasis.ByblockingCXCR4,amajorcoreceptorforHIV-1entry,CXCL12actsasanendogenousinhibitorofCXCR4-tropicHIV-1strains.CXCL12wasshowntobeexpressedinmanytissuesinmiceincludingbrain,thymus,heart,lung,liver,kidney,spleenandbonemarrow.

DESCRIPTIONDESCRIPTION

Source:RecombinanthumanCXCL12aisproducedinE.coli(accessionno.

P48061-2).

ProteinSequence:KPVSLSYRCPCRFFESHVARANVKHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNK

MolecularMass:7.98kDabyMassSpec.

Purity:>97%

Activity:EC50=0.62nMdeterminedbyCalciumFluxwithhumanCXCR4cells.

SPABindingassayofCXCL12/SDF-1αtoCXCR7(IC50=14pM)andCXCR4(IC50=2nM).

EndotoxinLevel:<0.01EUper1ugofproteinbyLALmethod.

Form:Lyophilized.

CarrierProtein:None.

PREPARATIONANDSTORAGEPREPARATIONANDSTORAGE

Reconstitution:Recommendedat100ug/mlinsteriledistilledwater.

StABIlityandStorage:12monthsfromdateofreceipt,-20oCto-70oC,assupplied.

1month,2oCto8oC,understerileconditionsafterreconstitution.

3months,-20oCto-70oC,understerileconditionsafterreconstitution.

Forinvitroinvestigationaluseonly.Notforuseindiagnosticortherapeuticprocedures.

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