RecombinantCCL2(MCP-1),biotinylated

ORDERINGINFORMATIONORDERINGINFORMATION

CatalogNo.Size

50186PB-22ug

50186PB-1010ug

50186PB-5050ug

50186PB-100100ug

BACKGROUNDBACKGROUND

CCL2,alsoknownasMonocyteChemoattractantProtein-1(MCP-1)isasmallcytokine(~13kDa)thatbelongstotheCCchemokinefamily.CCL2recruitsmonocytes,memoryTcells,anddendriticcellstositesofinflammationproducedbyeithertissueinjuryorinfection.Itisanchoredintheplasmamembraneofendothelialcellsbyglycosaminoglycansidechainsofproteoglycans.Tobecomeactivated,CCL2proteinhastobecleavedbymetalloproteinaseMMP-12.CCL2isimplicatedinthepathogenesisofseveraldiseasescharacterizedbymonocyticinfiltrates,suchaspsoriasis,rheumatoidarthritisandatherosclerosis.CCR2andCCR4aretwocellsurfacereceptorsthatbindCCL2.

DESCRIPTIONDESCRIPTION

Source:RecombinanthumanCCL2isproducedinE.coli(accessionno.

P13500,aa24-99).

Modification:Biotinylated

ProteinSequence:QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSIDHLDKQTQTPKT

MolecularMass:11.084kDabyMassSpec.

Purity:>97%bySDS-PAGE

Activity:EC50=0.25-1.2nMdeterminedbyMigrationAssayofrecombinantCCR2-expressingcells.

EndotoxinLevel:<0.01EUper1ugofproteinbyLALmethod.

Form:Lyophilized.

CarrierProtein:None.

PREPARATIONANDSTORAGEPREPARATIONANDSTORAGE

Reconstitution:CentrifugetubepriortoresUSPending.Recommendedat100ug/mlinsteriledistilledwater.

StABIlityandStorage:12monthsfromdateofreceipt,-20oCto-70oC,assupplied.

1month,-20oCto-70oC,understerileconditionsafterreconstitution.Bestifusedimmediatelyafterreconstitution.

Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseindiagnosticortherapeuticprocedures.

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