productdatasheet

RecombinantRatFGF-7(KeratinocyteGrowthFactor)

ORDERINGINFORMATION

CatalogNo.:45102

Size:20ug

SPECIFICATIONSUMMARY

Source:Genetically-engineeredE.coli.MolecularWeight:~17,000daltonsPurity:>90%

Diluent:PBS/1.5MNaCl

Preservatives:None

PURIFICATION

Purifiedbyheparinaffinitychroma-tography.

BIOLOGICALACTIVITY

TheactivityofratFGF-7at1-10ng/mlisdeterminedbytritiatedthymidineincorporationusingculturedmousekeratinocytes.Maximumstimulationobservedat1ng/ml.Theoptimalconcentrationofthisproductfor

usewithothercelltypesmustbedeterminedbytheenduser.

SPECIFICITY

45102bindstokeratinocytesandmostparenchymalepithelialcells;bindsspecificsplicevariantofFGFreceptorexpessedinepithelialcells(FGFr2IIIb).

DILUTIONINSTRUCTIONS

Dilutewitheither0.1%TFA/40%acetonitrileorPBS/0.1%CHAPSforbeststABIlityandtopreventabsorptionofproductonwallsofvessels.

STORAGEANDSTABILITY

Thisproductisstableforatleastone(1)yearat-20oCto-70oC.Keepfrozenuntiluse.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.

REFERENCES

Yan,G.(1991)InVitroCellDevBiol27A:437;Wang,F.etal(1995)JBiolChem270:10222.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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Phone858.675.2405

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