productdatasheet

RecombinantHumanVascularEndothelialGrowthFactor

CatalogNumber	Size
45109P-2	2ug
45109P-10	10ug
45109P-1000	1000ug

CatalogNumber	Size
45109P-2	2ug
45109P-10	10ug
45109P-1000	1000ug

ORDERINGINFORMATION

Format:Sterilefiltered,lyophilized.

Purification:Chromatographic

DESCRIPTION

RecombinantHumanVEGFproducedin

E.coliisadouble,non-glycosylated,polypeptidechaincontaining165aminoacidswithamolecularmassof38,231daltons.ThisproducthasfullBIOLOGicalactivity.

SPECIFICATIONSUMMARY

Source:EscherichiacoliStABIlizers:NonePreservatives:None

Purity:Greaterthan98%byRP-HPLC,anion-exchangechromatography;reducingandnon-reducingSDS-PAGE,silverstain.

Dimers/Aggregates:<1%bySDS-PAGE

BiologicalActivity:Thebiologicalactivityisdeterminedbythedose-dependentstimulationofthe

proliferationofhumanumbilicalveinendothelialcellsusingaconcentrationof1.0to8.0ng/ml.

Endotoxin:<0.1ng/ug

ProteinContent:Determinedspectrophotometricallyat280nm(extinctioncoefficient0.2875)andbyRP-HPLCanalysisagainstacalibratedstandard.

SOLUBILITY

Reconstitutethelyophilizedproductinsterilewaternotlessthan100ug/ml.

STORAGEANDSTABILITY

Lyophilizedproductisstableatroomtemperaturefor3weeks.Storelyophilizedproductdesiccatedat-20°C.Uponreconstitution,storeat4°Cfor2-7daysoraliquotandstoreat-20°C.Forlong-termstorageaddeitherHSAorBSAto0.1%.Avoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures

QEDBioscience,Inc.10919TechnologyPlace,SuiteC

SanDiego,CA92127

TollFree800.929.2114

Phone858.675.2405

Fax858.592.1509

info@qedbio.com

Visitourwebsiteforadditionalproductinformationandtoorderonline.

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