productdatasheet

Anti-DNMT3LMonoclonalAntibody

ORDERINGINFORMATION

CatalogNo.:12530(cloneS117-9)Size:100uginPBS,pH7.4;50%glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.

BACKGROUND

MethylationofDNAatcytosineresiduesisimportantinregulationofgeneexpression.HypermethylationofCpGislandsintumorsuppressorgenesorhypomethylationofbulkgenomicDNAmaybelinkedtodevelopmentofcancer.ThreefamiliesofmammalianDNAmethyltransferasegeneshavebeenidentified:DNMT1,DNMT2,andDNMT3.TheDNMT3familymembers,DNMT3aandDNMT3b,arestronglyexpressedinembryonicstem(ES)cells,buttheirexpressionisdownregulatedindifferentiatingEScellsandislowinadultsomatictissue.StudiesshowthatDNMT3LregulatestheactivityofDNMT3aandDNMT3bandstimulatestheircatalyticactivities.DNMT3Lhasbeenlinkedspecificallytotheprocessofcarcino-genesisthroughitsroleinnuclearprogramming.

SPECIFICATIONSUMMARYAntigen:Fusionprotein,aa1-387ofhumanDNMT3L(accessionno.

BAA95556).

HostSpecies:MouseAntibodyClass:IgG1

SPECIFICITY

ThisantibodyrecognizeshumanDNMT3L.Itdoesnotcross-reactwithDNMT3aorDNMT3b.

APPLICATIONS

Immunoblotting:useat1-10ug/ml.Abandof~45kDaisdetected.

ImmunohistochemistryandImmunocytochemistry:useat0.1-1ug/mlImmunofluorescence:useat1-10ug/mlThesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.

Positivecontrol:LysateofCOScellstransientlytransfectedwithFlag-taggedDNMT3L.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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SanDiego,CA92127

TollFree800.929.2114

Phone858.675.2405

Fax858.592.1509

info@qedbio.com

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