DNMT1MonoclonalAntibody

ORDERINGINFORMATION

CatalogNo.:12595(clone4G11-C7)Size:100uginPBS(2mg/ml),pH7.4,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.

BACKGROUND

MethylationofDNAatcytosineresiduesisimportantinregulationofgeneexpressionandgenomicimprinting.Hyper-methylationofCpGislandsintumorsuppressorgenesorhypomethylationofbulkgenomicDNAmaybelinkedtodevelopmentofcancer.ThusfarthreefamiliesofmammalianDNAmethyl-transferasegeneshavebeenidentified:DNMT1,DNMT2,andDNMT3.DNMT1is

constitutivelyexpressedinproliferatingcells,andinactivationofthisgenecausesglobaldemethylationofgenomicDNAandembryoniclethality.DNMT1co-purifieswiththeretinoblastoma(Rb)tumorsuppressorgeneproductE2F1andwithHDAC1.DNMT1cooperateswithRbtorepresstranscriptionfrompromoterscontainingE2F-bindingsiteswhichsuggestsalinkbetweenDNAmethylation,histonedeacetylaseandsequence-specificDNAbindingactivityaswellasagrowth-regulatorypathwaythatisdisruptedincancercells.

SPECIFICATIONSUMMARY

Antigen:Syntheticpeptidecorrespondingtoaa637-650ofhumanDNMT1(accessionno.NP_001370).HostSpecies:Mouse

AntibodyClass:IgG2b

SPECIFICITY

Thisantibodyrecognizeshuman,mouse,andZebrafishDNMT1.

APPLICATIONS

ELISA

Immunoblotting:useat2ug/ml.Abandof

~190kDaisdetected.

Thesearerecommendedconcentrations;endusershoulddetermineoptimalconcentrationsfortheirapplications.

Positivecontrols:ESandH1299celllysates.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.

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