DicerMonoclonalAntibody

ORDERINGINFORMATIONCatalogNo.:12532(cloneS167-7)Size:100uginPBS(1mg/ml),pH7.4,50%glycerol,0.09%sodiumazide.

PurifiedbyProteinGaffinitychromatography.

BACKGROUND

DicerisamemberoftheRNaseIIIfamilythatspecificallycleavesdouble-strandedRNAstogeneratemicroRNAs(miRNAs).Afterlongprimarytranscriptpri-miRNAsareprocessedtostem-loopedpre-miRNAsbyDrosha,pre-miRNAsaretransportedtothecytoplasmandfurtherprocessedbyDicertoproduce22-nucleotidematuremiRNAs.ThematuremiRNAthenbecomesapartoftheRNA-InducedSilencingComplex(RISC)andcanbindtothe3'UTRofthetargetmRNA.

SPECIFICATIONSUMMARY

Antigen:Fusionproteincorrespondingtoaa1638-1899ofmouseEndoribonucleaseDicer(accessionno.Q8R418).

HostSpecies:MouseAntibodyClass:IgG1SPECIFICITY

Thisantibodyrecognizeshuman,mouseandratDicer.

APPLICATIONS

Immunoblotting:useat1ug/ml.Predictedmolecularweightis~215kDa.

#12532onratbrainlysate.

PositiveControl:ratbrainlysate.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.

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