productdatasheet

MouseSpermProteinsp56MonoclonalAntibodies

ORDERINGINFORMATION

CatalogNo.:55101thru55105Size:100ugProteinG-purifiedantibodyinPBS,pH7.4.

SPECIFICATIONSUMMARY

Antigen:Mixtureofnativeandformaldehyde-zincfixedspermpurifiedthroughaPercollgrADIent.

HostSpecies:MouseAntibodyClass:55105=IgG3;55101thru55104=IgG1.

StABIlizers:NonePreservatives:None.Availableonrequest.

SPECIFICITYANDACTIVITY

Theseantibodiesspecificallyrecognizemousespermproteinsp56.Theywillreactwithdenatured,nonreducedsp56butnotwithdenatured,reducedsp56.Theseantibodiesdonotcross-reactwithhumansperm.Relativeantibodytitersforantibodybindingtosolidphasesp56:55101(7C5)=66pM,55102(5F12)=4nM,55103(4B2)=29nM,55104(7H12)=70nM,55105(12D2)

=400nM.

APPLICATIONS

Theseantibodiesmaybeusedinimmunohistochemistrytolocalizesp56onmousespermandinimmunoblotsorELISAtoidentifysp56.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Theseantibodiesarestableforatleastone(1)yearat-20oCto-70oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.

REFERENCE

Chengetal.(1994)JCellBiol125:867;

Bookbinderetal.(1995)Science269:

86.

Forinvitroinvestigationaluseonly.

Notforuseintherapeuticordiagnosticprocedures.

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