TATA-BindingProtein(TBP)MonoclonalAntibody

ORDERINGINFORMATION

CatalogNo.:70102(clone1TBP18).Size:200ugProteinG-purifiedantibodyinPBS,pH7.4.

BACKGROUND

TheTATA-bindingprotein(TBP)playsacentralroleintheassemblyofmosteukaryotictranscriptioninitiationcomplexes.TBPassembleswithotherproteinstoformuniquemultimericcomplexesforeachofthethreedifferentnuclearRNApolymerases.

TBPhasbeenclonedfromavarietyofspeciesandconsistsoftwodistinctdomains.TheC-terminaldomain(~180aminoacids)

ishighlyconservedamongspecies,whereastheN-terminaldomainvariesconsiderablyinlengthandsequenceamongdifferentspecies.TheconservedC-terminaldomaincontainstheDNA-bindingregionaswellasregionsthatinteractwithpositiveand

negativeregulatoryproteins.InhumanTBP,thenon-conservedN-terminaldomainincludesastretchofglutamineresiduesrangingfrom26to42.SeveralresearchgroupshavefoundanassociationbetweenexpansionofthispolyglutaminetractwithrareformsofspinocerebellarataxiaandotherneurodegenerativediseasesincludingHuntington’sdisease.

SPECIFICATIONSUMMARY

Antigen:RecombinanthumanTBP.

HostSpecies:Mouse.

AntibodyClass:IgG1.

Preservatives:None.Availableonrequest.

SPECIFICITY

Thisantibodyrecognizesanepitopewithinaminoacidresidues1-20ofhuman,mouseandratTBP.ItdoesnotreactwithTBPfromDrosophila,yeast,silkworm,orXenopus.Otherspeciesnotinvestigated.

APPLICATIONS

ThisantibodymaybeusedinWesternblotandelectrophoreticmobility(gelshift)assaystodetectmammalianTBP.ItmayalsobeusedtoimmunoprecipitateatruncatedTBPmoleculecontainingonlytheN-terminaldomain.

1. ReactivitywithrhuTBPandHeLacellnuclearextractwithMAb#70102at2ug/ml.

2. EpitopemappingbyimmunoblotwithfragmentsofrhuTBPwithMAB#70102at2ug/ml.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone

(1)yearat-20oCto-70oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.

PRODUCTREFERENCE

ThompsonNEetal.2004ProteinExpressionandPurification36:186-197.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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