productdatasheet
CatalogNo.:70104(N3H10)and70105(N9C1).
Size:200ugProteinGpurifiedantibodyinPBS,pH7.4.
Antigen:Proximalsequenceelement(PSE1)ofthehumanU1smallnuclearRNAextractedfromhumanplacenta.HostSpecies:Mouse.
AntibodyClass:70104=IgG2b;70105=IgG1.
Preservatives:None.Availableonrequest.
70104recognizesthesmallβsubunit(~70kDa)and70105recognizesthelargeαsubunit(~80-90kDa)ofplacentalPSE1andhumanKuinsolutionandwhenboundtoDNA.
APPLICATIONSTheseantibodiesworkwellinWesternblotandelectrophoreticmobility(gelshift)assaysforidentifyingPSE1orKu.Theymaybeusedtoimmuno-depleteKuproteinfromproteinmixturespriortospecificDNAaffinitychromatography.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Theseantibodiesarestableforatleastone(1)yearat-20oCto-70oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.
KnuthMWetal.1990.JBiolChem
265:17911-17920.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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