ORDERINGINFORMATION
CatalogNo.:43057
Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.
BACKGROUND
Mutationsinp95/NBS1geneareassociatedwithNijmegenbreakagesyndrome,anautosomalrecessivechromosomalinstABIlitysyndromecharacterizedbymicrocephaly,growthretardation,immunodeficiency,andcancerpredisposition.TheencodedproteinisamemberoftheMRE11/RAD50double-strandbreakrepaircomplexwhichconsistsof5proteins.ThisgeneproductisthoughttobeinvolvedinDNAdouble-strandbreakrepairandDNAdamage-inducedcheckpointactivation.
SPECIFICATIONSUMMARY
Antigen:Peptidesequencethatincludesphosphorylationsiteofserine343(S-L-S(p)-Q-G)derivedfromhumanp95/NBS1.
HostSpecies:Rabbit
Accessionno.:O60934,NP_002476.2
Specificity:Thisantibodydetectsendogenoushumanp95/NBS1onlywhenphosphorylatedatserine343.
APPLICATIONS
Immunoblotting:useatdilutionof1:500-1:1,000.Abandof~95kDaisdetected.
Detectionofp95/NBS1(phospho-Ser343)inextractsofHeLacellsuntreatedortreatedwithhydroxyurea.
Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplication.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.
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