ORDERINGINFORMATION
CatalogNo.:43013
Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.
BACKGROUND
NFκBisatranscriptionfactorfoundinalmostallcelltypesandisinvolvedinmanyBIOLOGicalprocessessuchasinflammation,immunity,differentiation,cellgrowth,tumOrigenesisandapoptosis.NFκBiscontrolledbyvariousmechanismsofpost-translationalmodificationandsubcellularcompartmentalizationaswellasbyinteractionswithothercofactorsorcorepressors.suchasp65-p50andp65-c-Relcomplexes.TheNF-kappa-Bp65-p65complexappearstobeinvolvedininvasin-mediatedactivationofIL-8expression.
SPECIFICATIONSUMMARY
Antigen:Peptidesequencethatincludesphosphorylationsiteofserine468derivedfromhumanNFκB-p65andconjugatedtoKLH.
Accessionno.:Q04206,NP_001138610.1
HostSpecies:Rabbit
Specificity:Thisantibodydetectsendogenoushuman,mouse,andratNFκB-p65onlywhenphosphorylatedatserine468.
APPLICATIONS
Westernblotting:useatdilutionof1:500-1:1,000.
Abandof~65kDaisdetected.
Immunofluorescence:useatdilutionof1:100-1:200.
DetectionofNFκB-p65(phospho-Ser468)inHeLacellextracts.
DetectionofNFκB-p65(phospho-Ser468)inmethanol-fixedHeLacells.
Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.
QED Bioscience为您的所有生物学研究需求提供大量定制服务，例如定制抗体生产，细胞培养服务，细胞系开发和细胞库。我们的定制抗体服务包括多克隆和单克隆抗体生产，体外抗体生产和抗体纯化。
