ORDERINGINFORMATION
CatalogNo.:43004
Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.
BACKGROUND
Elk-1isamemberoftheEtsfamilyoftranscriptionfactorsandtheternarycomplexfactor(TCF)subfamily.Proteinsofthatsubfamilyformaternarycomplexbybindingtotheserumresponsefactorandtheserumresponseelementinthepromoterofthec-fosprotooncogene.Elk-1proteinisanucleartargetfortheras-raf-MAPKsignalingcascade.
SPECIFICATIONSUMMARY
Antigen:Peptidesequencethatincludesphosphorylationsiteofserine383(T-L-S(p)-P-I)derivedfromhumanElk-1andconjugatedtoKLH.
Accessionno.:P19419,NP_001107595.1
HostSpecies:Rabbit
Specificity:Thisantibodydetectsendogenoushuman,mouse,andratElk-1onlywhenphosphorylatedatserine383.
APPLICATIONS
Westernblotting:useatdilutionof1:500-1:1,000.Abandof~47kDaisdetected.
Immunohistochemistry:useatdilutionof1:50-1:100.
DetectionofElk-1(Phospho-Ser383)inHT29cellextractsuntreatedortreatedwithUV.
Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.
DetectionofElk-1(phospho-Ser383)inparaffin-embeddedhumanbreastcarcinomatissue.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.
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