ORDERINGINFORMATION
CatalogNo.:43064
Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.
BACKGROUND
Bcl-2proteinsuppressesapoptosisinavarietyofcellularsystemsincludingfactor-dependentlymphohematopoieticandneuralcells.ItregulatescelldeathbycontrollingthemitochondrialmembranepermeABIlityandappearstofunctioninafeedbackloopsystemwithcaspase.Bcl-2inhibitscaspaseacativityeitherbypreventingreleaseofcytochromecfromthemitochondriaand/orbybindingtoapoptosis-activatingfactor(Apaf-1).ThepredictedmolecularweightofBcl-2is~26kDa.
SPECIFICATIONSUMMARY
Antigen:Peptidesequencethatincludesphosphorylationsiteofthreonine56(G-H-T(p)-P-H)derivedfromhumanBcl-2andconjugatedtoKLH.
HostSpecies:Rabbit
Specificity:ThisantibodydetectsendogenoushumanBcl-2onlywhenphosphorylatedatthreonine56.
Accessionno.:P10415,NP_000624.2
APPLICATIONS
Immunohistochemistry:useatdilutionof1:50-1:100.
DetectionofBcl-2(phospho-Thr56)inparaffin-embeddedhumanbreastcarcinomatissue.
Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplication.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.
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