ORDERINGINFORMATION
CatalogNo.:43031
Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.
BACKGROUND
ATF2isatranscriptionfactorthatisamemberoftheleucinezipperfamilyofDNAbindingproteinthatcanperformmultiplefunctions.ItcanbindtothecAMP-responsiveelement(CRE),anditcanformahomodimeroraheterodimerwithc-JunandstimulateCRE-dependenttranscription.ATF2isalsoahistoneacetyltransferase(HAT)thatspecificallyacetylateshistonesH2BandH4invitro.ATF2mayalsobeinvolvedinacell'sDNAdamageresponseindependentofitsroleintranscriptionalregulation.
SPECIFICATIONSUMMARY
Antigen:Peptidesequencethatincludesphosphorylationsitesofthreonine71or53(T-P-T(p)-P-T)derivedfromhumanATF2andconjugatedtoKLH.
Accessionno.:P15336,NP_001871.2
HostSpecies:Rabbit
Specificity:Thisantibodydetectsendogenoushuman,mouse,andratATF2onlywhenphosphorylatedatthreonine71or53.
APPLICATION
Immunoblotting:useatdilutionof1:500-1:1,000.Abandof~65-75kDaisdetected.
Immunohistochemistry:useatdilutionof1:50-1:100.
DetectionofATF2(phospho-Thr71or53)inextractsofHUVECcellsuntreatedortreatedwithAnisomycin.
DetectionofATF2(phospho-Thr71or53)inparaffin-embeddedhumanbreastcarcinomatissue.
Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.
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