ORDERINGINFORMATION
CatalogNo.:43055
Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.
BACKGROUND
Akt,alsoknownasAkt1andProteinkinaseB(PKB),isaserine/threonine-specificproteinkinasethatplaysakeyroleinmultiplecellularprocessessuchasglucosemetabolism,apoptosis,cellproliferation,transcriptionandcellmigration.Akt1isalsoabletoinduceproteinsynthesispathwaysandis,therefore,akeysignalingproteininthecellularpathwaysthatleadtoskeletalmusclehypertrophy,andgeneraltissuegrowth.Sinceitcanblockapoptosis,andtherebypromotecellsurvival,Akt1hasbeenimplicatedasamajorfactorinmanytypesofcancer.
SPECIFICATIONSUMMARY
Antigen:Peptidesequencethatincludesphosphorylationsitesofserinethreonine308(M-K-T(p)-F-C)derivedfromhumanAktandconjugatedtoKLH.
Accessionno.:P31749,NP_001014431.1
HostSpecies:Rabbit
Specificity:Thisantibodydetectsendogenoushuman,mouse,andratAktonlywhenphosphorylatedatthreonine308.
APPLICATION
Immunoblotting:useatdilutionof1:500-1:1,000.Abandof
~60kDaisdetected.
Immunohistochemistry:useatdilutionof1:50-1:100.
Immunofluorescence:useatdilutionof1:100-1:200.
DetectionofAkt(phospho-Thr308)inextractsofHeLacellsuntreatedortreatedwithEGF,serumorboth.
DetectionofAkt(phospho-Thr308)inparaffin-embeddedhumanlungcarcinomatissue.
DetectionofAkt(phospho-Thr308)inmethanol-fixedHeLacellsshowingnucleardotstaining.
Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.
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