LibraryPackNo.100ug/clone
180101All4clones
LibraryPackNo.100ug/clone
180101All4clones
ORDERINGINFORMATION
CatalogNo.CloneNo.MAbSubtypeSize
18001TGEV10-116.3.3IgG1100ug,500ug
18002TGEV11-314.6IgG1100ug,500ug
18003TGEV15-886.6IgG2a100ug,500ug
18004TGEV15-948.2.1IgG2a100ug,500ug
Format:PurifiedantibodyinPBS,pH7.4
BACKGROUND
TransmissibleGastroenteritis(TGEV)isanenvelopedCoronaviruswithapositive-sensesingle-strandedRNAgenomeandahelicalsymmetry.Thegenomicsizeofcoronavirusesisapproximately28.6kilobases.ProteinsthatcontributetotheoverallstructureofTGEVincludethespike(S),envelope(E),membrane(M)andnucleocapsid(N).OthercoronavirusesbelongingtothisfamilyincludeFelinecoronavirus(FeCV),Caninecoronavirus(CCV),andFelineinfectiousperitonitisvirus(FIPV).TGEVinfectspigs.However,inyoungpigs,themortalityrateiscloseto100%.ThepathologyofTGEVissimilartothatofothercoronaviruses.Coronavirusesenterthehostbyfirstattachingtothehostcellusingthespikeglycoprotein.TheSproteininteractswiththeporcineaminopeptidaseN(pAPN),acellularreceptor,toaideinitsentry.AdomainintheSspikeproteinisrecognizedbypAPN,andtransfectionofpAPNoccurstononpermissivecellsandinfectsthemwithTGEV.Oncethevirusinfectsthehost,itmultipliesinthecellliningofthesmallintestineresultinginthelossofabsorptivecellsthatinturnleadstoshorteningofvilli.TheinfectedswinethenhasreducedcapABIlityfordigestingfoodanddiefromdehydration.
SPECIFICATIONSUMMARY
Antigen:TGEV-infectedSTcells
HostSpecies:Mouse
Specificity:TheseantibodiesrecognizeTGEVanddonotcross-reactwithFIPV,FeCV,orCCV.
#18003and#18004canneutralizeTGEVinvitro.
APPLICATIONS
TheseantibodieshavebeenqualifiedforuseinELISAtodetectTGEV.#18003and#18004maybeusedinTGEVneutralizationassays.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Theseantibodiesarestableforatleastone(1)yearat-20oto-70oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notintendedforuseintherapeuticordiagnosticprocedures.
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