ORDERINGINFORMATION
CatalogNo.:18758
Format:100ugProteinG-purifiedantibodyinPBS,pH7.4.
BACKGROUND
Picornavirusesaresmall,non-envelopedRNAviruseswithanicosahedralcapsidandasinglestrand,plus-senseRNAgenome.Thegenomeencodesasinglepolyproteinthatisproteolyticallyprocessedbyviralproteasesintostructuralandnon-structuralproteins.Thefamilyofpicornavirusesincludesnumeroushumanandanimalvirusesincludingmorethan100SEROtypesofhumanrhinoviruses(HRV).HRVinfectionsarecharacteristicupperairwayinfections(themaincauseofthecommoncold),andtheyprovokesignificantlowerairwaysymptomsforpatientswithasthma,cysticfibrosis,orchronicobstructivepulmonarydisease.
SPECIFICATIONSUMMARY
Antigen:PurifiedHRV16.
Cloneno.:R16-7
HostSpecies:Mouse
AntibodySubtype:IgG2b
SPECIFICITY
Thisnon-neutralizingantibodyrecognizescapsidproteinVP2(mw~30kDa)ofHRV16,HRV1A,andHRV39andVP2precursorsVP0(mw~37kDa)andP1(mw~90kDa).
APPLICATIONS
Immunoblotting:useat2-5ug/ml.Immunohistochemistry:useat1-10ug/ml.
LysatesofHeLacellsinfectedwithRVserotypes1A,2,14,16or49andblottedwith#18758.
Thesearerecommendedconcentrations;endusersshoulddetermineoptimalconcentrationsfortheirapplications.
IHCstainingforviralcapsidproteinwith#18758:(A)UninfectedHeLacells,(B)HRV16-infectedHeLacells,
(C)Negativebronchialbiopsysection,(D)Positivebronchialbiopsysection.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.
PRODUCTREFERENCES
MosserAGetal.2002JInfectDis185:734.
MosserAGetal.2005AmJRespirCritCareMed171:645.
JurgeitAeta.2010VirologyJ7:264.
ChattorajSSetal.2011InfectImmun79:4131.
Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticprocedures.
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