TRAP1/Hsp75MonoclonalAntibody

ORDERINGINFORMATION

CatalogNo.:11136(cloneTrap1-6)Size:100uginPBS(1mg/ml),pH7.4,50%glycerol,0.09%sodiumazide.

PurifiedbyProteinGaffinitychromatography.

BACKGROUND

Themolecularchaperonefamilyof90kDaheatshockproteins(Hsp90)isahighlyconservedproteinfamilythatplaysanimportantphysiologicalrole.Hsp90isbestknownforitsassociationwithsignaltransductionprocesses.Tumornecrosisfactorreceptor-associatedprotein1(TRAP1)isarecentlyclonedhomologofHsp90.LikeHsp90,TRAP1isassociatedwithnumerousproteinsthatareinvolvedinmultiplepathways.DataindicatesthatTRAP1isamitochondrialmatrixprotein:itislocalizedinmitochondriaofmammaliancellsandcontainsamitochondrialtargetingpresequence.

SPECIFICATIONSUMMARY

Antigen:RecombinanthumanTRAP1

HostSpecies:Mouse

AntibodyClass:IgG2a

SPECIFICITY

ThisantibodyrecognizeshumanTRAP1.Otherspecieshavenotbeeninvestigated.

APPLICATIONS

Immunoblotting:useat1ug/ml.Abandof

~75kDaisdetected.Immunocytochemistry:useat0.1-1.0ug/ml.Immunofluorescence:useat1-10ug/ml.

Positivecontrol:HeLacellsorlysateThesearerecommendedconcentrations;endusershoulddetermineoptimalconcentrationsfortheirapplications.

LocalizationofTRAP1tomitochondriainPC-3Mcells.(A)StainingofmitochondriawithMitoTrackerdye;(B)StainingofnucleiwithDAPI;

(C)Immunofluorescentstainingofmitochondriawithproductno.11134;(D)tricolorcompositeofimagesA-C.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.

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