ORDERINGINFORMATION
CatalogNo.Size
18864P100ug
Format:1mg/mlantibodyconjugatedtohorserADIshperoxidase(HRP)inPhosphateBufferedSaline(PBS),pH7.2,0.2%BSA,0.1%Pro-Clean400.Molarenzyme/antibodyratio=4:1.
BACKGROUND
TheT7tagisan11aminoacidpeptide(MASMTGGQQMG)derivedfromtheleadersequenceofT7bacteriophagegene10whichencodesaT7majorcapsidprotein.TheT7tagservesasataginmanyexpressionvectors,andantibodiesspecificfortheT7tagareusefulforidentifyingandisolatingT7-taggedproteins.
SPECIFICATIONSUMMARY
Antigen:T7(MASMTGGQQMG)conjugatedtoKLH.
HostSpecies:Rabbit
Specificity:Antibodywasaffinity-purifiedonthepeptide.
APPLICATIONS
Immunoblotting:useatadilutionof1:1,000-1:30,000.
Immunocytochemistry:useatadilutionof1:200-1:500.
ELISA:useatadilutionof1:10,000-1:100,000.
Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.
DetectionofT7-taggedfusionproteinin200,100,and50ngofE.colilysatewith#18864Pdiluted1:5,000.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat2-8oC.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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