ORDERINGINFORMATION
CatalogNo.Size
188491mg
18849-01100ug
Format:1mg/mlinPhosphateBufferedSaline(PBS),pH7.2,0.09%sodiumazide.
BACKGROUND
TheHAepitopetagisderivedfromtheHA-moleculecorrespondingtoaminoacidsYPYDVPDYAofhumaninfluenzahemagglutinin.Itisfrequentlyusedasanepitopetaginexpressionvectors.ManyrecombinantproteinshavebeenengineeredtoexpresstheHAtag,whichdoesnotappeartointerferewiththebioactivityorthebiodistributionoftherecombinantprotein.
SPECIFICATIONSUMMARY
Antigen:HA(YPYDVPDYA)conjugatedtoKLH.
HostSpecies:Goat
Specificity:Antibodywasaffinity-purifiedonthepeptide.
APPLICATIONS
Immunoblotting:useatadilutionof1:10,000-1:25,000.
Immunocytochemistry:useatadilutionof1:100-1:400.
Immunoprecipitation:use2-10ug/mglysate.ELISA:fordetectionuseatadilutionof1:1,000-1:30,000;forcoatinguseatadilutionof1:100-1:500.
Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.
DetectionofHA-taggedfusionproteinin200,67,22,7,and2ngofE.colicelllysatewith18849diluted1:25,000.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat2-8oC.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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