ORDERINGINFORMATION
CatalogNo.Size
188501mg
18850-01100ug
Format:1mg/mlinPhosphateBufferedSaline(PBS),pH7.2,0.09%sodiumazide.
BACKGROUND
TheHAepitopetagisderivedfromtheHA-moleculecorrespondingtoaminoacidsYPYDVPDYAofhumaninfluenzahemagglutinin.Itisfrequentlyusedasanepitopetaginexpressionvectors.ManyrecombinantproteinshavebeenengineeredtoexpresstheHAtag,whichdoesnotappeartointerferewiththebioactivityorthebiodistributionoftherecombinantprotein.
SPECIFICATIONSUMMARY
Antigen:HA(YPYDVPDYA)conjugatedtoKLH.
HostSpecies:Rabbit
Specificity:Antibodywasaffinity-purifiedonthepeptide.
APPLICATIONS
Immunoblotting:useatadilutionof1:1,000-1:20,000.
Immunocytochemistry:useatadilutionof1:100-1:400.
Immunoprecipitation:use1-4ug/mglysate.ELISA:fordetectionuseatadilutionof1:1,000-1:30,000;forcoatinguseatadilutionof1:100-1:500.
Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.
DetectionofHA-taggedfusionproteinin200,100,and50ngofE.colicelllysatewith18850diluted1:25,000.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat2-8oC.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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