ORDERINGINFORMATION
CatalogNo.Size
188421mg
18842-01100ug
Format:1mg/mlinPhosphateBufferedSaline(PBS),pH7.2,0.09%sodiumazide.
BACKGROUND
AGST-tagisoftenusedtoseparateandpurifyproteinsthatcontaintheGST-fusionprotein.Thetagis220aminoacidswhich,comparedtotagssuchasthemyc-tag,isquitelarge.ItcanbefusedtoeithertheN-terminusorC-terminusofaprotein.ManycommerciallyavailablesourcesofGST-taggedplasmidsincludeathrombindomainforcleavageoftheGSTtagduringproteinpurification.
SPECIFICATIONSUMMARY
Antigen:Glutathione-S-Transferase(GST)fromSchistosomajaponicum.
HostSpecies:Goat
Specificity:Antibodywasaffinity-purifiedonGST.
APPLICATIONS
Immunoblotting:useatadilutionof1:1,000-1:20,000.Immunocytochemistry:useatadilutionof1:100-1:400.Immunoprecipitation:use1-4ug/mglysate.
ELISA:fordetectionuseatadilutionof1:1,000-1:30,000;forcoatinguseatadilutionof1:100-1:500.
Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat2-8oC.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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