ORDERINGINFORMATION
CatalogNo.Size
18838P100ug
Format:1mg/mlconjugatedtohorserADIshperoxidase(HRP)inPhosphateBufferedSaline(PBS),0.2%BSA,0.1%Pro-Clean400.Molarenzyme/antibodyratio=4:1.
BACKGROUND
TheGlu-GlutagrepresentsthepeptidesequenceEYMPMEwhichisderivedfromthemiddleTantigenofmousepolyomavirus.Giventhesmallsizeofthispeptide,itisunlikelythatmodifyingproteinswithitwillaffectproteinstructureorfunction.
SPECIFICATIONSUMMARY
Antigen:Syntheticpeptidecorrespondingtoaa314-319(EYMPME)ofmiddleTantigenofmousepolyomavirusconjugatedtoKLH.
HostSpecies:Rabbit
Specificity:Antibodywasaffinity-purifiedonthepeptide.
APPLICATIONS
Immunoblotting:useatadilutionof1:1,000-1:30,000.
Immunocytochemistry:useatadilutionof1:200-1:500.
ELISA:useatadilutionof1:10,000-1:100,000.
Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.
DetectionofGlu-Glu-taggedproteininE.colilysatecontaining200,100,and50ngoftaggedproteinwith#18838Pdiluted1:5,000.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat2-8oC.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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