ORDERINGINFORMATION
Catalognos.:42024(clonePC5-5)and42043(clonePC8-7)
Format:100ugIgGin10mMPBS,pH7.4,1%BSA,lyophilized.
BACKGROUND
Collagens(typesI,II,III,IV,andV)aresynthesizedasprecursormoleculescalledprocollagens.Thesecontainadditionalpeptidesequences,the“propeptides”,attheamino-andcarboxy-terminalends.Propeptidesfacilitatethewindingofprocollagenmoleculesintoatriple-helixconformationwithintheendoplasmicreticulum.Thesepropeptidesarecleavedfromthecollagentriplehelixduringitssecretionafterwhichthecollagenspolymerizeintoextracellularcollagenfibrils.Therefore,theamountoffreepropeptidescorrelatesdirectlywithcollagenmoleculesynthesis.ProcollagenTypeIC-peptide(PIP)hasbeenextensivelyreferencedinstudiesthatcorrelatedcollagenlevelsanddisorderssuchasbonedisease,alcoholicliverdisease,livercirrhosis,andscirrhous(BorrmanntypeIV)adenocarcinomaofthestomach.
SPECIFICATIONSUMMARY
Antigen:HumanProcollagenTypeIC-Peptide
HostSpecies:Mouse
AntibodyClass:IgG1
Specificity:Theseantibodiesrecognizenon-denaturedhuman,bovine,canine,andequineProcollagenTypeIC-peptide.TheydonotreactwithratorrabbitProcollagenType1C-peptide.
APPLICATIONS
ELISA:0.2ug/mlwithsolidphaseantigen.ForsandwichELISA,use42043onthesolidphaseandlabeled42024fordetectionofbinding.
WesternBlot:10ug/mlundernon-reducingandnon-heatingconditions.
Immunohistochemistry:10ug/mlonculturedcells,frozenandparaffin-embeddedtissuesections(Antigenretrieval:ProteinaseKtreatment).
DILUTIONINSTRUCTIONS
Dissolvelyophilizedantibodyin50ulofdistilledwater(finalconcentration2.0mg/ml).
STORAGEANDSTABILITY
Thisproductdoesnotcontainpreservative.Thestocksolution(2.0mg/ml)canbestoredinaliquotsat-20oCfor1yearorcanbestoredat4oCfor6monthsafteradding0.1%sodiumazide.Dilutionsofstocksolutionshouldnotbestored.Avoidrepeatedfreeze-thawcycles.
PRODUCTREFERENCES
AngXMetal.MacromolecularCrowdingAmplifiesADIpogenesisofHumanBoneMarrow-DerivedMesenchymalStemCellsbyEnhancingthePro-AdipogenicMicroenvironment.TissueEngineeringPartAVolume20:966,2014.Westernblotting
GorurA.etal.COPII-coatedmembranesfunctionastransportcarriersofintracellularprocollagenI.JCellBiolApril20,2017,PMID28428367.Immunofluorescence
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.
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