LAMP2MonoclonalAntibody

ORDERINGINFORMATION

CatalogNo.:56272(cloneGL2A7,ref.GrangerBLetal.1990,JBiolChem265:12036-12403)Format:100uginPBS,pH7.4,purifiedbyProteinGaffinitychromatography,0.09%sodiumazide,50%glycerol.

BACKGROUND

Lysosome-associatedmembraneproteins(LAMP1andLAMP2)aremajorconstituentsofthelysosomalmembrane.Thesetwoproteinshavecloselyrelatedstructureswith37%sequencehomology.Botharetransmembraneglycoproteinslocalizedprimarilyinlysosomesandlateendosomes.LAMP2hasalsobeendetectedattheplasmamembraneofcellsundergoingdifferentiationandactivationandincellsthatsecretelysosomalhydrolases.CellsurfaceLAMP1andLAMP2promoteadhesionofhumanperipheralbloodmononuclearcells(PBMC)tovascularendotheliumwhichsuggeststhattheLAMPproteinsareinvolvedinadhesionofPBMCtositesofinflammation.DefectsinLAMP2areassociatedwithDanondisease.

SPECIFICATIONSUMMARY

Antigen:Gluteraldehyde-fixedmouseliverlysosomesHostSpecies:RatAntibodyClass:IgG1Specificity:Thisantibodyrecognizeshuman,mouse,andrabbitLAMP2(~100-110kDa).Accessionno.:NP_001017959.1GeneID:16784

APPLICATIONS

Immunoblotting:Useat1-2ug/ml.Abandof~100-110kDa(glycosylated)isdetected.

Immunofluorescence:Useat1-2ug/ml.ThisantibodylabelslysosomesandlateendosomesincellspermeABIlizedwithsaponin.

Lane1:MWladder,Lane2:20ugHEK293lysate,Lane3:10ug3T3NIHlysate

Thesearerecommendedconcentrations.

Distributionoflysosomesincirculatingendothelialcells(CEC)

Endusershoulddetermineoptimalconcentrationsfortheirapplication.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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