productdatasheet

RPA14MonoclonalAntibody

ORDERINGINFORMATION

CatalogNo.:56215

Size:100ugProteinG-purifiedantibodyinPBS,pH7.4

SPECIFICATIONSUMMARY

Antigen:N-terminally6x-Histaggedfusionproteincorrespondingtofull-lengthhumanRPA14expressedin

E.coli.

HostSpecies:MouseAntibodyClass:IgG1/κStABIlizers:None

Preservatives:None.Availableonrequest.

SPECIFICITY

ThisantibodyrecognizeshumanandmouseRPA14.RPA(ReplicationProteinA)isasingle-strandedDNAbindingprotein.HumanRPAisaheterotrimericproteincontainingsubunitsof70,32,and14kD.ThisproteincomplexishighlyconservedineukaryotesandisessentialinDNAreplication,homologousrecombination,andnucleotideexcisionrepair.

APPLICATIONS

Immunoblotting,immunoprecipitation:useat0.2-1ug/ml.

Positivecontrol:HeLa,Raji,and3T3cellsandrecombinantprotein.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone

(1)yearat-70oC.Avoidmultiplefreeze-thawcycles.

REFERENCES

Iftode,Cetal.1999.CritRevBiochemMolBiol34:141-180;Bochkarev,Aetal.1999.EMBO18:4498-4504;Dong,

Jetal.1999.BiochemJ337:311-317;Sibenaller,ZAetal.1998.Biochemistry37:12496-12506;Stigger,

Eetal.1998.JBiolChem273:9337-

9343.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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