Anti-MLLN-TerminusMonoclonalAntibody

ORDERINGINFORMATIONCatalogno.:34041Size:100ugProteinG-purifiedantibodyinPBS,pH7.4.

BACKGROUND

MLL(MixedLineageLeukemia)isaproto-oncogenethatismutatedinavarietyofacuteleukemias.MLLisproteolyticallyprocessedinto2fragments,MLLNandMLLC,thatdisplayoppositetranscriptionalproperties.ProcessedMLLNandMLLC

associatewitheachotherviaN-terminalandC-terminalintramolecularinteractiondomains.MLLprocessingoccursrapidlyaftertranslationandisfollowedby

phosphorylationofMLLC.MLLNdisplays

transcriptionalrepressionactivity,whereasMLLChasstrongtranscriptionalactivationproperties.Leukemia-associatedMLLfusionproteinslacktheMLLprocessingsites,donotundergocleavage,andareunabletointeractwithMLLC.These

observationssuggestthatposttranslationalmodificationsofMLLmaybeinvolvedinregulatingMLLactivityasatranscriptionfactor,andthisaspectofitsfunctionisperturbedbyleukemogenicfusions.

SPECIFICATIONSUMMARYAntigen:Maltose-bindingfusionproteincontainingMLLaminoacids161-356(Uniprot#Q03164).Cloneno.:N4.4Host:MouseAntibodyClass:IgG1Preservatives:None-availableonrequest.

SPECIFICITY

ThisantibodyrecognizeshumanandmouseMLLN.

APPLICATIONS

Immunoblotting:useat2ug/ml.Abandof

~300kDaisdetected.

K562cellnuclearextractwith#34041at2ug/ml.

Immunoprecipitation:use2-10ugper500ul(1-3mg/ml)ofcelllysate.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

Positivecontrol:K562celllysate.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

PRODUCTREFERENCES

YokoyamaAetal.2002Blood100:3710-

3718.

YokoyamaAetal.2005Cell123:207-218.

Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.

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