productdatasheet

YersiniapestisVAntigenMonoclonalAntibodies

CatalogNumber	Size
18772-200	200ug
18773-200	200ug
18774-200	200ug
18775-200	200ug
18776-200	200ug
18772-1000	1mg
18773-1000	1mg
18774-1000	1mg
18775-1000	1mg
18776-1000	1mg

CatalogNumber	Size
18772-200	200ug
18773-200	200ug
18774-200	200ug
18775-200	200ug
18776-200	200ug
18772-1000	1mg
18773-1000	1mg
18774-1000	1mg
18775-1000	1mg
18776-1000	1mg

ORDERINGINFORMATION

Format:TheseantibodiesarepurifiedbyProteinGaffinitychromatographyandaresuppliedinPBS,pH7.4,containing0.1%sodiumazideaspreservative.

BACKGROUND

Y.pestis,thecausativeagentofplague,haslow-Ca+2response(LCR)virulenceplasmidsthatencodeasetofsecretedvirulenceproteinsincludingYersiniaouterproteins(YOPS)andtheVantigen,LcrV.SomeoftheYOPSplayaroleinadherencetoeukaryoticcellswhileothersaretargetedintoaeukaryoticcellatthesiteofadherence.TheYOPSderangecellularsignalingandcytoskeletalfunctionsnecessaryforhostdefensessuchasphagocytosis.LcrVisasecretedanti-hostcomponentwithdirectimmunomodulatoryeffects.LcrVexpressionisinvolvedintargetingofYOPStohostcellsandisrequiredforcytotoxicity.

SPECIFICATIONSUMMARY

Antigen:Recombinantfull-length

Y.pestisVantigen.

HostSpecies:MouseAntibodyClass:AllareIgG1.Preservative:0.1%NaN3

SPECIFICITY

Theseantibodiesspecificallyrecognize

Y.pestisVantigen.Theydonotcross-reactwithY.pestiscapsularF1antigen.

APPLICATIONS

Immunoblotting:End-usershoulddetermineoptimalconcentration.Werecommendtestingaconcentrationrangeof1-10ug/ml.Abandof37kDaisdetected.

ELISA:End-usershoulddetermineoptimalconcentrationfortheirsample.SandwichELISA:use18772ascoatingantibodyand18773asdetectantibody.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone

(1)yearat4oCor-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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SanDiego,CA92127

TollFree800.929.2114

Phone858.675.2405

Fax858.592.1509

info@qedbio.com

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