productdatasheet

BacillusanthracisProtectiveAntigenMonoclonalAntibody

ORDERINGINFORMATION

CatalogNos.:18720(C3),18721

(101),18722(102),18723(103),18724

(104),18725(105),and18726(106).

Size:200ugpurifiedantibodyinPBS,pH7.4.

BACKGROUND

AnthraxtoxinproducedbyBacillusanthracisiscomposedofthreeproteins:ProtectiveAntigen(PA),EdemaFactor(EF),andLethalFactor(LF).Toxicityrequirestheassemblyofallthreeoftheseproteins,andthisprocessisinitiatedwhenfull-lengthPAbindstothecellsurfaceATR(anthraxtoxinreceptor).Afterbinding,PAisenzymaticallycleavedtoyielda63kDfragmentwhichthenbindsEFandLF,andthecomplexisinternalized.

SPECIFICATIONSUMMARY

Antigen:PurifiedB.anthracis

ProtectiveAntigen(83kD).

HostSpecies:Mouse

AntibodyClass:IgG1=18720,18725,18726;IgG2b=18721,18722,18723,

18724

Preservative:0.1%NaN3

SPECIFICITY

Theseantibodiesrecognizedifferentepitopesofthe83kDand63kDformsofB.anthracisPA.Theydonotcross-reactwithB.anthracisEF,LF,orspore/vegetativesurfaceantigens.

APPLICATIONS

Immunoblotting:use18721,18723,

18725,or18726at5ug/ml.

SandwichELISA:usecoatingantibody(18720or18725)at5ug/ml;usedetect

antibody(18722,18723,or18726)at1ug/ml.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Theseantibodiesarestableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

QEDBioscience,Inc.10919TechnologyPlace,SuiteC

SanDiego,CA92127

TollFree800.929.2114

Phone858.675.2405

Fax858.592.1509

info@qedbio.com

Visitourwebsiteforadditionalproductinformationandtoorderonline.

qedbio.com

QED Bioscience为您的所有生物学研究需求提供大量定制服务，例如定制抗体生产，细胞培养服务，细胞系开发和细胞库。我们的定制抗体服务包括多克隆和单克隆抗体生产，体外抗体生产和抗体纯化。