Anti-PUMA(CT)Antibody

ORDERINGINFORMATION

CatalogNo.:11007

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

BACKGROUND

PUMA(p53UpregulatedModulatorofApoptosis)isapro-apoptoticBcl-2familymemberthatencodestwoBH3domain-containingproteins,PUMA-andPUMA-.PUMAproteinsbindBcl-2,localizetomitochondria,andinducecytochromecreleaseandapoptosisinresponsetop53.

SPECIFICATIONSUMMARY

Antigen:Syntheticpeptidecorrespondingtoaa180-193ofhuman(accessionnoNP_055232).ThissequenceisidenticalinhumanPUMA-andPUMA-.HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide

SPECIFICITY

ThisantibodyrecognizeshumanandmousePUMA(23kDa).Alowermolecularweightbandat16kDaisdetectedinMOLT4andU937celllysatesandprobablyrepresentsthePUMA-protein.

APPLICATIONS

Immunoblotting:useat2ug/ml.

WesternblotanalysisofPUMAinhuman(H)K562andmouse(M)NIH/3T3celllysateswithPUMAantibodyat2μg/ml.

Positivecontrol:K562celllysate.

Immunocytochemistry:useat1ug/ml.

ImmunocytochemicalstainingofPUMAinK562cellswithPUMAantibodyat1μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

QED Bioscience为您的所有生物学研究需求提供大量定制服务，例如定制抗体生产，细胞培养服务，细胞系开发和细胞库。我们的定制抗体服务包括多克隆和单克隆抗体生产，体外抗体生产和抗体纯化。